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固定在与2,4-二氯苯氧乙酸偶联的琼脂糖上的蛋白质对豌豆和玉米DNA的RNA转录的刺激作用。

Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.

作者信息

Venis M A

出版信息

Proc Natl Acad Sci U S A. 1971 Aug;68(8):1824-7. doi: 10.1073/pnas.68.8.1824.

DOI:10.1073/pnas.68.8.1824
PMID:4256895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389301/
Abstract

When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.

摘要

当豌豆或玉米芽的粗提物通过与合成植物激素2,4-二氯苯氧乙酸衍生物偶联的琼脂糖柱时,某些蛋白质组分被保留,而未修饰的琼脂糖不吸附蛋白质。适当洗脱可得到一种蛋白质因子,该因子能使依赖DNA的RNA合成(由大肠杆菌聚合酶支持)提高40%-200%。初步证据表明,该因子可能影响RNA链的起始。

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引用本文的文献

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
EVIDENCE FOR A RING STRUCTURE OF POLYOMA VIRUS DNA.多瘤病毒DNA环状结构的证据。
Proc Natl Acad Sci U S A. 1963 Aug;50(2):236-43. doi: 10.1073/pnas.50.2.236.
3
On the similarity of plant and animal histones.论植物组蛋白与动物组蛋白的相似性。
Biochemistry. 1966 Aug;5(8):2563-70. doi: 10.1021/bi00872a012.
4
Microbial synthesis and degradation of indole-3-acetic acid. 3. The isolation and characterization of indole-3-acetyl-epsilon-L-lysine.吲哚-3-乙酸的微生物合成与降解。3. 吲哚-3-乙酰基-ε-L-赖氨酸的分离与特性鉴定。
Biochemistry. 1968 Feb;7(2):601-5. doi: 10.1021/bi00842a013.
5
Stimulation of chromatin-bound RNA polymerase activity by a soluble factor.一种可溶性因子对染色质结合的RNA聚合酶活性的刺激作用。
Biochim Biophys Acta. 1970 Dec 14;224(2):667-70. doi: 10.1016/0005-2787(70)90609-x.
6
A protein intermediary in the interaction of a hormone with the genome.一种在激素与基因组相互作用中起中介作用的蛋白质。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):897-903. doi: 10.1073/pnas.63.3.897.
7
Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.赤霉素对离体豌豆细胞核中RNA合成的促进作用。
Proc Natl Acad Sci U S A. 1968 Jan;59(1):269-76. doi: 10.1073/pnas.59.1.269.
8
A receptor molecule for estrogens: studies using a cell-free system.雌激素的一种受体分子:使用无细胞系统的研究
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1740-3. doi: 10.1073/pnas.57.6.1740.
9
Selective enzyme purification by affinity chromatography.通过亲和色谱法进行选择性酶纯化。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. doi: 10.1073/pnas.61.2.636.
10
A factor mediating interaction of kinins with the genetic material.
Biochim Biophys Acta. 1970 Feb 18;199(2):511-8. doi: 10.1016/0005-2787(70)90093-6.