固定在与2,4-二氯苯氧乙酸偶联的琼脂糖上的蛋白质对豌豆和玉米DNA的RNA转录的刺激作用。
Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.
作者信息
Venis M A
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1824-7. doi: 10.1073/pnas.68.8.1824.
Abstract
When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.
摘要
当豌豆或玉米芽的粗提物通过与合成植物激素2,4-二氯苯氧乙酸衍生物偶联的琼脂糖柱时，某些蛋白质组分被保留，而未修饰的琼脂糖不吸附蛋白质。适当洗脱可得到一种蛋白质因子，该因子能使依赖DNA的RNA合成（由大肠杆菌聚合酶支持）提高40%-200%。初步证据表明，该因子可能影响RNA链的起始。
相似文献
1
Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.固定在与2,4-二氯苯氧乙酸偶联的琼脂糖上的蛋白质对豌豆和玉米DNA的RNA转录的刺激作用。
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1824-7. doi: 10.1073/pnas.68.8.1824.
2
The molecular mechanism of the enzymic reaction in RNA synthesis.
Biochim Biophys Acta. 1967 May 30;138(3):480-98. doi: 10.1016/0005-2787(67)90545-x.
3
Kinetics of the initiation and growth of RNA chains at high and low ionic strengths.
Mol Biol. 1971 Jan-Feb;5(1):126-32.
4
Transcription of mitochondrial DNA in vitro from Neurospora crassa.粗糙脉孢菌线粒体DNA的体外转录
Eur J Biochem. 1971 Aug 25;21(4):478-88. doi: 10.1111/j.1432-1033.1971.tb01493.x.
5
Factors and rifampicin influencing RNA polymerase isolated from chromatin of eukaryotic cell.影响从真核细胞染色质中分离出的RNA聚合酶的因素及利福平
Biochem Biophys Res Commun. 1970 Sep 10;40(5):1194-200. doi: 10.1016/0006-291x(70)90922-8.
6
Termination of RNA transcription on the replicative form DNA of bacteriophage fd.噬菌体fd复制型DNA上RNA转录的终止
J Mol Biol. 1971 Nov 28;62(1):81-8. doi: 10.1016/0022-2836(71)90132-x.
7
In vitro transcription of a transfer RNA gene.转运RNA基因的体外转录
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1260-6. doi: 10.1073/pnas.66.4.1260.
8
Escherichia coli RNA polymerase: purification, subunit structure, and factor requirements.大肠杆菌RNA聚合酶：纯化、亚基结构及因子需求
Fed Proc. 1970 May-Jun;29(3):1164-9.
9
Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue.2,4-二氯苯氧乙酸对大豆下胚轴组织中染色质RNA聚合酶的增强作用。
Biochim Biophys Acta. 1968 Nov 20;169(1):35-43. doi: 10.1016/0005-2787(68)90006-3.
10
A factor mediating interaction of kinins with the genetic material.
Biochim Biophys Acta. 1970 Feb 18;199(2):511-8. doi: 10.1016/0005-2787(70)90093-6.
引用本文的文献
1
[The effect of auxin and cytokinin on RNA-synthesis in sterile tobacco tissue].[生长素和细胞分裂素对无菌烟草组织中RNA合成的影响]
Planta. 1974 Mar;118(1):7-16. doi: 10.1007/BF00390498.
2
Isolation of two protein factors from maize involved in poly U-dependent polyphenylalanine synthesis by maize ribosomes.从玉米核糖体参与多聚 U 依赖性多苯丙氨酸合成的过程中分离出两种蛋白质因子。
Planta. 1976 Jan;131(1):47-50. doi: 10.1007/BF00387344.
3
Ribonucleic acid synthesis by chromatin isolated from Phaseolus aureus Roxb. : The effect of endogenous ribonuclease.从 Phaseolus aureus Roxb. 分离的染色质的核糖核酸合成：内源性核糖核酸酶的影响。
Planta. 1977 Jan;137(2):153-7. doi: 10.1007/BF00387552.
4
Characterization of a cytoplasmic auxin receptor from tobacco-pith callus.从烟草髓愈伤组织中鉴定细胞质生长素受体。
Planta. 1980 Jun;149(1):44-7. doi: 10.1007/BF00386225.
5
Enhancement of RNA polymerase activity by a factor released by auxin from plasma membrane.生长素从质膜释放的一种因子对RNA聚合酶活性的增强作用。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3146-50. doi: 10.1073/pnas.69.11.3146.
6
Chirality and stereochemical recognition in DNA-phytohormone interactions: a model approach.DNA-植物激素相互作用中的手性与立体化学识别：一种模型方法。
Orig Life. 1978 Sep;9(1):7-15. doi: 10.1007/BF00929709.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
EVIDENCE FOR A RING STRUCTURE OF POLYOMA VIRUS DNA.多瘤病毒DNA环状结构的证据。
Proc Natl Acad Sci U S A. 1963 Aug;50(2):236-43. doi: 10.1073/pnas.50.2.236.
3
On the similarity of plant and animal histones.论植物组蛋白与动物组蛋白的相似性。
Biochemistry. 1966 Aug;5(8):2563-70. doi: 10.1021/bi00872a012.
4
Microbial synthesis and degradation of indole-3-acetic acid. 3. The isolation and characterization of indole-3-acetyl-epsilon-L-lysine.吲哚-3-乙酸的微生物合成与降解。3. 吲哚-3-乙酰基-ε-L-赖氨酸的分离与特性鉴定。
Biochemistry. 1968 Feb;7(2):601-5. doi: 10.1021/bi00842a013.
5
Stimulation of chromatin-bound RNA polymerase activity by a soluble factor.一种可溶性因子对染色质结合的RNA聚合酶活性的刺激作用。
Biochim Biophys Acta. 1970 Dec 14;224(2):667-70. doi: 10.1016/0005-2787(70)90609-x.
6
A protein intermediary in the interaction of a hormone with the genome.一种在激素与基因组相互作用中起中介作用的蛋白质。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):897-903. doi: 10.1073/pnas.63.3.897.
7
Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.赤霉素对离体豌豆细胞核中RNA合成的促进作用。
Proc Natl Acad Sci U S A. 1968 Jan;59(1):269-76. doi: 10.1073/pnas.59.1.269.
8
A receptor molecule for estrogens: studies using a cell-free system.雌激素的一种受体分子：使用无细胞系统的研究
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1740-3. doi: 10.1073/pnas.57.6.1740.
9
Selective enzyme purification by affinity chromatography.通过亲和色谱法进行选择性酶纯化。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. doi: 10.1073/pnas.61.2.636.
10
A factor mediating interaction of kinins with the genetic material.
Biochim Biophys Acta. 1970 Feb 18;199(2):511-8. doi: 10.1016/0005-2787(70)90093-6.
Zotero 插件