转运RNA基因的体外转录
In vitro transcription of a transfer RNA gene.
作者信息
Daniel V, Sarid S, Beckmann J S, Littauer U Z
出版信息
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1260-6. doi: 10.1073/pnas.66.4.1260.
Abstract
The Escherichia coli tRNA(Tyr) gene carried by the varphi80psu(3) + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA(Tyr)-like polynucleotide chains were detected by competition with purified E. coli(32)P-tRNA(Tyr) for specific hybridization sites on varphi80psu(3) + DNA. Analysis by sucrose gradient centrifugation showed the tRNA(Tyr)-like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor rho during the transcription did not significantly reduce the average size of the in vitro synthesized tRNA(Tyr)-like chains.
摘要
由φ80psu(3)+转导噬菌体携带的大肠杆菌tRNA(Tyr)基因在体外由依赖DNA的RNA聚合酶转录。通过与纯化的大肠杆菌(32)P-tRNA(Tyr)竞争φ80psu(3)+DNA上的特异性杂交位点，检测酶促合成的类似tRNA(Tyr)的多核苷酸链。蔗糖梯度离心分析表明，类似tRNA(Tyr)的链在沉降系数方面具有异质分布，在8S左右有一个宽峰。转录过程中终止因子rho的存在并没有显著降低体外合成的类似tRNA(Tyr)链的平均大小。
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