Feldt-Rasmussen U, Hyltoft Petersen P, Date J
Acta Endocrinol (Copenh). 1979 Mar;90(3):440-50. doi: 10.1530/acta.0.0900440.
The aim of the present investigation was to describe variations in serum thyroglobulin in relation to sex and age in a group of normal persons. The method used was a modified double antibody radioimmunoassay characterized by pre-incubation at 37 degrees C of standard or sample with antiserum, resulting in a reduced total incubation time. Both sensitivity and precision were comparable to other published methods. Of the 152 blood-donors initially investigated, 7 were excluded due to the presence of antithyroglobulin antibodies as evidenced by a radioassay. Both sexes were equally represented with an even distribution of ages from 20-65 years. Increased serum thyroglobulin with increasing age was demonstrated, the correlation being significant in women (Kendall's tau, P less than 0.001). Detectable concentrations of serum thyroglobulin (above 1.7 microgram/1) were found in 94%. Based on the logarithmic transformation, the upper reference limits were determined for men less than or equal to 40 years: 36 microgram/l, greater than 40 years: 44 microgram/l (difference between groups not significant, P greater than 0.05), and for women less than or equal to 40 years: 30 microgram/l, greater than 40 years: 60 microgram/l (significant difference, P less than 0.005).
本研究的目的是描述一组正常人血清甲状腺球蛋白随性别和年龄的变化情况。所采用的方法是改良的双抗体放射免疫测定法，其特点是标准品或样品与抗血清在37℃下预温育，从而缩短了总的温育时间。灵敏度和精密度与其他已发表的方法相当。在最初研究的152名献血者中，有7人因放射测定法证明存在抗甲状腺球蛋白抗体而被排除。男女比例相同，年龄分布在20至65岁之间且均匀。结果表明，血清甲状腺球蛋白随年龄增长而升高，在女性中相关性显著（肯德尔tau系数，P<0.001）。94%的人血清甲状腺球蛋白浓度可检测到（高于1.7微克/升）。基于对数转换，确定了男性≤40岁的参考上限：36微克/升，>40岁：44微克/升（两组间差异不显著，P>0.05），以及女性≤40岁：30微克/升，>40岁：60微克/升（差异显著，P<0.005）。
