Bona C, Anteunis A, Robineaux R, Halpern B
Clin Exp Immunol. 1972 Nov;12(3):377-90.
Behaviour of normal and hydrolase-treated guinea-pig lymph node lymphocytes versus heterologous ALS was studied by means of biophysical and immunological techniques i.e. electrophoretic mobility, leucoagglutination, cytotoxicity and visualization of antigen–antibody reaction in electron microscopy. Our results clearly revealed that heterologous ALS antibodies reacted with mannose and sialic acid containing glycoproteins located on the lymphocyte surface. Sialic acid and other main constituents of the lymphocyte cell coat, i.e. the sulphated mucopolysaccharides have an important role in the electronegative charges of the cell and in binding of the ALS antibodies. β-Glycosidase as well as α-maltase treatment of the lymph node lymphocyte increased from 45% to 100% the reactivity to ALS as revealed by the peroxidase reaction in electron microscopy. These results suggest that all lymph node lymphocytes can react with ALS antibodies, but that the reactive sites of 45% of these cells are masked by glucidic sub-units.
运用生物物理和免疫学技术，即电泳迁移率、白细胞凝集、细胞毒性以及电子显微镜下抗原 - 抗体反应的可视化，研究了正常和经水解酶处理的豚鼠淋巴结淋巴细胞对异种抗淋巴细胞血清（ALS）的反应。我们的结果清楚地表明，异种ALS抗体与位于淋巴细胞表面的含甘露糖和唾液酸的糖蛋白发生反应。唾液酸和淋巴细胞细胞膜的其他主要成分，即硫酸化粘多糖，在细胞的负电荷以及ALS抗体的结合中起重要作用。如电子显微镜下的过氧化物酶反应所示，对淋巴结淋巴细胞进行β - 糖苷酶以及α - 麦芽糖酶处理后，其对ALS的反应性从45%提高到了100%。这些结果表明，所有淋巴结淋巴细胞都能与ALS抗体发生反应，但这些细胞中有45%的反应位点被糖类亚基所掩盖。
