Margni R A, Hajos S
Immunology. 1973 Mar;24(3):435-43.
Methods for isolation and purification of precipitating and non-precipitating guinea-pig antibodies are described. The physicochemical properties of γ and γ non-precipitating antibodies are similar to γ and γ precipitating ones. Biological properties are also similar excepting the reverse Arthus reaction, which is positive with the precipitating and negative with the non-precipitating antibodies. Bivalence of these antibodies was experimentally demonstrated. Precipitating antibodies K do not differ greatly from those obtained with the corresponding non-precipitating ones. The incapacity to precipitates with the antigen may be a consequence of a steric impediment.
描述了分离和纯化豚鼠沉淀性和非沉淀性抗体的方法。γ和γ非沉淀性抗体的物理化学性质与γ和γ沉淀性抗体相似。除反向阿瑟斯反应外,生物学性质也相似,沉淀性抗体的反向阿瑟斯反应为阳性,而非沉淀性抗体为阴性。通过实验证明了这些抗体的二价性。沉淀性抗体K与相应的非沉淀性抗体获得的抗体差异不大。不能与抗原沉淀可能是空间位阻的结果。