Hougland A E, Gaush C R, Mayberry W R
Biochem J. 1979 Jan 1;177(1):311-7. doi: 10.1042/bj1770311.
Glycolipid and fatty acid compositions were studied in whole cells and plasma membranes from two dog kidney cell lines (Madin-Darby and SV40-transformed cells) grown in monolayer and suspension cultures. Glycolipids, which account for 5% or less of the total lipids in dog kidney cells, were substantially increased in plasma membranes relative to whole cells. Sialoglycolipids more complex than a Tay-Sachs-like ganglioside were not found in any whole-cell or plasma-membrane preparation of this study. Dog kidney cells transformed by SV40 virus contained primarily a less complex sialoglycolipid, haematoside. Neutral glycolipids comprised 26-43% of the total glycolipid content in Madin-Darby preparations, whereas in transformed cells and membranes neutral glycolipids constituted only 1-22% of the total glycolipid content. Ceramide trihexoside was found in Madin-Darby cultures, but not in transformed cultures. The values for short-chain fatty acids from neutral glycolipids and for saturated fatty acids were generally higher than the values for these fatty acids in calf serum.
研究了在单层培养和悬浮培养条件下生长的两种犬肾细胞系（麦迪逊-达比犬肾细胞和SV40转化细胞）的全细胞和质膜中的糖脂和脂肪酸组成。糖脂在犬肾细胞总脂质中占比5%或更少，相对于全细胞，其在质膜中的含量显著增加。在本研究的任何全细胞或质膜制剂中均未发现比泰-萨克斯样神经节苷脂更复杂的唾液酸糖脂。经SV40病毒转化的犬肾细胞主要含有一种不太复杂的唾液酸糖脂——血苷脂。在麦迪逊-达比制剂中，中性糖脂占总糖脂含量的26% - 43%，而在转化细胞和质膜中，中性糖脂仅占总糖脂含量的1% - 22%。在麦迪逊-达比培养物中发现了神经酰胺三己糖苷，但在转化培养物中未发现。中性糖脂中的短链脂肪酸和饱和脂肪酸的值通常高于小牛血清中这些脂肪酸的值。
