哺乳动物染色体中DNA的复制：复制片段的分离
Replication of DNA in mammalian chromosomes: isolation of replicating segments.
作者信息
Taylor J H
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1083-7. doi: 10.1073/pnas.70.4.1083.
Abstract
From Chinese hamster cells, blocked at the beginning of the S phase by treatment with fluorodeoxyuridine and pulse-labeled with [(3)H]thymidine, one can release segments of native DNA containing template polyuncleotide chains and the most recently replicated DNA. The segments, which can be released without shear by lysis at temperatures below the melting point for the double helix, can be separated from both the nascent single chains (Okazaki pieces) and the long strands of native DNA in sodium perchlorate gradients. These 26S segments are equivalent to about 6 mum of native DNA, but may be replication forks rather than linear pieces.
摘要
用氟脱氧尿苷处理中国仓鼠细胞，使其在S期开始时被阻断，并用[³H]胸腺嘧啶核苷进行脉冲标记，这样就能释放出包含模板多核苷酸链和最新复制DNA的天然DNA片段。这些片段在低于双螺旋熔点的温度下通过裂解即可无剪切地释放出来，在高氯酸钠梯度中可与新生单链（冈崎片段）和天然DNA长链分离。这些26S片段相当于约6μm的天然DNA，但可能是复制叉而非线性片段。
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本文引用的文献
1
Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture.哺乳动物体细胞遗传学。II. 组织培养中细胞的染色体组成。
J Exp Med. 1958 Aug 1;108(2):259-68. doi: 10.1084/jem.108.2.259.
2
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Biochem Biophys Res Commun. 1969 Feb 7;34(3):291-300. doi: 10.1016/0006-291x(69)90830-4.
3
Buoyant density of DNA synthesized at different stages of S phase in Chinese hamster cells.中国仓鼠细胞S期不同阶段合成的DNA的浮力密度。
Exp Cell Res. 1971 Feb;64(2):481-4. doi: 10.1016/0014-4827(71)90106-6.
4
A unique property of the replicating region of chromosomal DNA.染色体DNA复制区域的一个独特特性。
Biochim Biophys Acta. 1966 Dec 21;129(3):651-4. doi: 10.1016/0005-2787(66)90088-8.
5
Programmed synthesis of deoxyribonucleic acid during the cell cycle.细胞周期中脱氧核糖核酸的程序性合成。
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6
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
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