DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.
作者信息
Okazaki R, Arisawa M, Sugino A
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7. doi: 10.1073/pnas.68.12.2954.
Abstract
In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally functions in filling gaps between the nascent short segments synthesized by the replication complex. Possible implications of the finding are discussed in relation to other abnormal properties of these mutants.
摘要
在缺乏DNA聚合酶I的大肠杆菌突变体中，新复制的短DNA连接的速率约为野生型菌株的10%。据推测，DNA聚合酶I通常在填补由复制复合体合成的新生短片段之间的间隙中发挥作用。结合这些突变体的其他异常特性，讨论了这一发现可能的影响。
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