Donoso J A, Haskins K M, Himes R H
Cancer Res. 1979 May;39(5):1604-10.
The influence of microtubule-associated proteins on the interaction of vincristine with microtubules and tubulin was examined. Microtubules containing associated proteins were prepared in vitro from tubulin preparations which contained the proteins or from pure 6S tubulin and isolated microtubule-associated proteins. The presence of the associated proteins caused microtubules to be converted to stable spiral structures upon reacting with vincristine. When the proteins were absent, spirals were not formed, and the microtubules were completely disassembled by vincristine. At 0 degrees, 6S tubulin was converted to amorphous aggregates by vincristine, whereas if the associated proteins were present spirals were formed.
研究了微管相关蛋白对长春新碱与微管及微管蛋白相互作用的影响。含有相关蛋白的微管是在体外由含有这些蛋白的微管蛋白制剂或由纯6S微管蛋白及分离出的微管相关蛋白制备而成。相关蛋白的存在使得微管在与长春新碱反应时转变为稳定的螺旋结构。当不存在这些蛋白时，不会形成螺旋结构，微管会被长春新碱完全拆解。在0摄氏度时，长春新碱会将6S微管蛋白转变为无定形聚集体，而如果存在相关蛋白则会形成螺旋结构。
