Musumeci V, Leone G, Bizzi B
J Clin Pathol. 1974 Oct;27(10):794-7. doi: 10.1136/jcp.27.10.794.
A sensitive immunological assay for plasminogen and/or plasmin was developed by using a haemagglutination inhibition technique. Plasma and the corresponding euglobulin fraction of healthy subjects and of patients with hyperfibrinolysis (liver cirrhosis, thrombolytic treatment) were assayed for plasminogen using immunological and caseinolytic techniques. In hyperfibrinolytic states discrepancies were found between immunoreactive and caseinolytic plasma plasminogen whereas a good correlation was observed between immunoreactive and caseinolytic euglobulin plasminogen. Experiments in vitro suggested that these discrepancies might depend on the presence in hyperfibrinolytic plasma of variable amounts of plasmin(ogen)-related antigen which has no caseinolytic activity and is not precipitated with euglobulin.
采用血凝抑制技术开发了一种用于检测纤溶酶原和/或纤溶酶的灵敏免疫测定法。使用免疫和酪蛋白溶解技术，对健康受试者以及纤溶亢进患者（肝硬化、溶栓治疗）的血浆和相应的优球蛋白组分进行纤溶酶原检测。在纤溶亢进状态下，免疫反应性血浆纤溶酶原与酪蛋白溶解活性之间存在差异，而免疫反应性与酪蛋白溶解活性的优球蛋白纤溶酶原之间观察到良好的相关性。体外实验表明，这些差异可能取决于纤溶亢进血浆中存在的可变数量的无酪蛋白溶解活性且不被优球蛋白沉淀的纤溶酶（原）相关抗原。
