Skilleter D N, Paine A J
Chem Biol Interact. 1979 Jan;24(1):19-33. doi: 10.1016/0009-2797(79)90100-5.
The relative toxicities of particulate beryllium phosphate, soluble beryllium sulphate and a beryllium sulphosalicylate complex to a rat liver parencymal derived cell line have been examined in culture. Due to the propensity of beryllium salts to form beryllium phosphate in solution the incubation medium used was free of inorganic phosphate. Cell death measured by the loss of cellular lactate dehydrogenase into the medium can be produced within 76 h from beryllium phosphate and beryllium sulphosalicylate or 48 h from beryllium sulphate provided the cells have, irrespective of the form of added beryllium, taken up a minimum of 2--5 nmol Be/10(6) cells. Whilst beryllium phosphate was readily taken up as a particle, beryllium complexed with excess sulphosalicylate was not so markedly accumulated by the cells except possibly by formation of small amounts of beryllium phosphate in the medium as a result of inorganic phosphate lost from the cells. The extent of beryllium uptake from beryllium sulphate quantitatively most resembled that observed for beryllium phosphate but was largely independent of beryllium phosphate formation in the medium and not accompanied by the uptake of the SO42- anion. However, the accumulation of beryllium derived from beryllium sulphate did appear to be associated with the production of a sedimentable from believed most probably to be colloidal beryllium hydroxide. The uptake of all forms of beryllium was temperature sensitive and metabolic inhibitor studies and treatment of the cells with trypsin or neuraminidase supported the view that the distinct behaviour of beryllium derived from beryllium sulphate may be related to the enhanced toxicity of this form both under the conditions used and when administered to experimental animals.
已在培养中研究了颗粒状磷酸铍、可溶性硫酸铍和铍磺基水杨酸盐复合物对大鼠肝实质衍生细胞系的相对毒性。由于铍盐在溶液中易于形成磷酸铍，因此所用的孵育培养基不含无机磷酸盐。如果细胞无论添加铍的形式如何，摄取的铍最少为2 - 5 nmol Be/10(6) 个细胞，则通过培养基中细胞乳酸脱氢酶的损失来测量的细胞死亡可在76小时内由磷酸铍和铍磺基水杨酸盐产生，或在48小时内由硫酸铍产生。虽然磷酸铍很容易作为颗粒被摄取，但与过量磺基水杨酸盐络合的铍没有被细胞如此明显地积累，除非可能由于细胞中无机磷酸盐的损失在培养基中形成少量磷酸铍。从硫酸铍摄取铍的程度在数量上最类似于观察到的磷酸铍，但在很大程度上与培养基中磷酸铍的形成无关，并且不伴随着SO42-阴离子的摄取。然而，源自硫酸铍的铍的积累似乎确实与一种可沉淀物质的产生有关，这种物质最可能是胶体氢氧化铍。所有形式的铍的摄取对温度敏感，代谢抑制剂研究以及用胰蛋白酶或神经氨酸酶处理细胞支持这样的观点，即在所用条件下以及给予实验动物时，源自硫酸铍的铍的独特行为可能与其增强的毒性有关。
