Dinsdale D
Br J Exp Pathol. 1982 Feb;63(1):103-8.
The fate of i.v. administered particles of beryllium phosphate (50 mumol/kg) has been investigated by electron microscopy in the liver of the rat. These particles were endocytosed by the Kupffer cells within 15 min of administration but they were never found in the endothelial or parenchymal cells of the liver. Endocytosed particles were rapidly degraded within vesicles which were identified as secondary lysosomes. Pronounced swelling of these lysosomes subsequently developed, within 60 min of administration, resulting in distension of the entire cell. This vacuolation was entirely restricted to the Kupffer cells, particularly those in the pericanalicular region of the hepatic lobules. Lysosomal swelling, or even rupture, probably resulted in the subsequent death of these cells by the intra-cytoplasmic release of beryllium.
通过电子显微镜研究了静脉注射磷酸铍颗粒（50微摩尔/千克）在大鼠肝脏中的命运。这些颗粒在给药后15分钟内被库普弗细胞内吞，但从未在肝脏的内皮细胞或实质细胞中发现。内吞的颗粒在被鉴定为次级溶酶体的小泡内迅速降解。给药后60分钟内，这些溶酶体随后出现明显肿胀，导致整个细胞扩张。这种空泡化完全局限于库普弗细胞，尤其是肝小叶小管周围区域的库普弗细胞。溶酶体肿胀甚至破裂，可能导致这些细胞因铍在细胞质内释放而随后死亡。
