Fraenkel D G, Horecker B L
J Bacteriol. 1965 Oct;90(4):837-42. doi: 10.1128/jb.90.4.837-842.1965.
Fraenkel, D. G. (Albert Einstein College of Medicine, New York, N.Y.), and B. L. Horecker. Fructose-1,6-diphosphatase and acid hexose phosphatase of Escherichia coli. J. Bacteriol. 90:837-842. 1965.-The conversion of fructose-1,6-diphosphate to fructose-6-phosphate (fructose-1,6-diphosphatase activity) is essential for growth of Escherichia coli on glycerol, acetate, or succinate, but is unnecessary for growth on hexoses or pentoses. It has sometimes been assumed that fructose-1,6-diphosphatase activity is due to a nonspecific acid hexose phosphatase. We have now obtained a number of one-step mutants which have lost the ability to grow on glycerol, succinate, or acetate, but which grow normally on hexoses; these mutants are deficient in a fructose-1,6-diphosphatase which can be assayed spectrophotometrically in the presence of Mg(++) and low concentrations of substrate. These mutants still possess the nonspecific acid hexose phosphatase, which does not require Mg(++) and is active only at much higher concentrations of fructose-1,6-diphosphate. Evidence is presented to support the hypothesis that the newly described activity is the physiological fructose-1,6-diphosphatase. The acid hexose phosphatase is a different enzyme whose function remains unknown.
弗伦克尔，D.G.（纽约市阿尔伯特·爱因斯坦医学院）和B.L.霍雷克。大肠杆菌的果糖-1,6-二磷酸酶和酸性己糖磷酸酶。《细菌学杂志》90:837 - 842。1965年。——果糖-1,6-二磷酸转化为果糖-6-磷酸（果糖-1,6-二磷酸酶活性）对于大肠杆菌在甘油、乙酸盐或琥珀酸盐上生长至关重要，但对于在己糖或戊糖上生长则不必要。有时人们认为果糖-1,6-二磷酸酶活性是由于一种非特异性酸性己糖磷酸酶。我们现在获得了一些一步突变体，它们失去了在甘油、琥珀酸盐或乙酸盐上生长的能力，但在己糖上能正常生长；这些突变体缺乏一种果糖-1,6-二磷酸酶，该酶可在存在Mg(++)和低浓度底物的情况下通过分光光度法进行测定。这些突变体仍然具有非特异性酸性己糖磷酸酶，该酶不需要Mg(++)，并且仅在高得多的果糖-1,6-二磷酸浓度下才有活性。提供了证据来支持新描述的活性是生理性果糖-1,6-二磷酸酶的假说。酸性己糖磷酸酶是一种不同的酶，其功能尚不清楚。
