四氮唑盐在脑膜肺炎电子传递研究中的应用。I. 还原型烟酰胺腺嘌呤二核苷酸系统。
Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system.
作者信息
Allen E G
出版信息
J Bacteriol. 1965 Dec;90(6):1505-12. doi: 10.1128/jb.90.6.1505-1512.1965.
Allen, Emma G. (Downstate Medical Center, Brooklyn, N.Y.). Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system. J. Bacteriol. 90:1505-1512. 1965.-Purified preparations of meningopneumonitis virus (MP) prepared from allantoic fluids of infected chick embryo reduce several tetrazolium salts in the presence of reduced nicotinamide adenine dinucleotide under both aerobic and anaerobic conditions. The pattern of reduction by MP differs from that seen in normal allantoic membrane homogenates, and is inhibited by several cations but not by KCN, atabrine, amytal, antimycin A, or 2,3-dimercaptopropanol (BAL). The reduction of cytochrome c by purified preparations of MP differs from its reduction of tetrazolium salts in that the cytochrome reaction is completely inhibited by BAL and partially inhibited by amytal, atabrine, and antimycin A. The cytochrome reductase of normal allantoic membrane preparations is completely inhibited by each of these compounds.
艾伦，艾玛·G.（纽约布鲁克林下州医学中心）。四氮唑盐在脑膜肺炎电子传递研究中的应用。I. 还原型烟酰胺腺嘌呤二核苷酸系统。《细菌学杂志》90:1505 - 1512。1965年。——从感染鸡胚尿囊液中制备的纯化脑膜肺炎病毒（MP）制剂，在有氧和无氧条件下，于还原型烟酰胺腺嘌呤二核苷酸存在时能还原几种四氮唑盐。MP的还原模式与正常尿囊膜匀浆中的不同，且受到几种阳离子的抑制，但不受氰化钾、阿的平、戊巴比妥、抗霉素A或2,3 - 二巯基丙醇（BAL）的抑制。纯化的MP制剂对细胞色素c的还原与其对四氮唑盐的还原不同，因为细胞色素反应完全被BAL抑制，部分被戊巴比妥、阿的平及抗霉素A抑制。正常尿囊膜制剂的细胞色素还原酶被这些化合物中的每一种完全抑制。
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