支原体对细胞培养中干扰素产生及干扰素检测的影响。
Effect of mycoplasma on interferon production and interferon assay in cell cultures.
作者信息
Armstrong D, Paucker K
出版信息
J Bacteriol. 1966 Jul;92(1):97-101. doi: 10.1128/jb.92.1.97-101.1966.
Abstract
Armstrong, D. (The Children's Hospital of Philadelphia, Philadelphia, Pa.), and K. Paucker. Effect of mycoplasma on interferon production and interferon assay in cell cultures. J. Bacteriol. 92:97-101. 1966.-The influence of mycoplasma on the production and action of interferon was studied in cultures of both L and human embryonic kidney (HEK) cells. Mycoplasma hominis 1, the Negroni agent, and the F12 mycoplasma were used for infection of L cells, and M. hominis 1 and M. pneumoniae for inoculation of HEK cells. All strains were capable of multiplication in the culture systems employed. None produced detectable levels of interferon, and responsiveness of the cells to induction of interferon by virus remained unaltered. Infection with mycoplasma did not impair the sensitivity of the cells to the action of interferon, nor was the replication of vesicular stomatitis virus noticeably diminished.
摘要
阿姆斯特朗，D.（费城儿童医院，宾夕法尼亚州费城），以及K.保克。支原体对细胞培养中干扰素产生及干扰素检测的影响。《细菌学杂志》92:97 - 101。1966年。——在L细胞和人胚肾（HEK）细胞培养物中研究了支原体对干扰素产生及作用的影响。人型支原体1、内格罗尼因子和F12支原体用于感染L细胞，人型支原体1和肺炎支原体用于接种HEK细胞。所有菌株都能在所采用的培养系统中繁殖。没有一种产生可检测水平的干扰素，并且细胞对病毒诱导干扰素的反应性保持不变。支原体感染并未损害细胞对干扰素作用的敏感性，水泡性口炎病毒的复制也没有明显减少。
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