Rinaldo C R, Overall J C, Cole B C, Glasgow L A
Infect Immun. 1973 Nov;8(5):796-803. doi: 10.1128/iai.8.5.796-803.1973.
A mycoplasmal species, Acholeplasma laidlawii, isolated as a contaminant from a fetal lamb kidney cell line, was shown to be associated with the induction of interferon in cultures of ovine peripheral blood leukocytes. Broth cultures of the mycoplasma induced between 20 and 230 U of interferon per ml in leukocytes from two adult ewes. The amount of interferon produced correlated with the inoculum size of mycoplasma. Interferon production was associated with replication of the mycoplasma in the leukocyte cultures. Interferon was not induced by sterile mycoplasmal broth, a cell-free filtrate of the mycoplasmal cultures, or heat-inactivated mycoplasmas. The antiviral substance was characterized as interferon by the usual criteria.
从胎羊肾细胞系中作为污染物分离出的一种支原体——莱氏无胆甾原体，被证明与绵羊外周血白细胞培养物中干扰素的诱导有关。该支原体的肉汤培养物在两只成年母羊的白细胞中每毫升诱导产生20至230单位的干扰素。产生的干扰素量与支原体的接种量相关。干扰素的产生与支原体在白细胞培养物中的复制有关。无菌支原体肉汤、支原体培养物的无细胞滤液或热灭活的支原体均未诱导产生干扰素。通过常规标准将这种抗病毒物质鉴定为干扰素。
