Fellowes O N
Appl Microbiol. 1966 Jan;14(1):86-91. doi: 10.1128/am.14.1.86-91.1966.
The inactivation of foot-and-mouth disease virus was studied by means of the interaction of neutral red, Toluidine Blue, and methylene blue with visible light. The virus, Type A, strain 1, CANEFA of Argentine origin, was grown in tissue culture and tested in the crude and clarified state. Virus and dye were mixed and incubated together at 4 C for 45 min in the dark, or were mixed and immediately exposed to the visible light source without prior incubation together. Mixtures of crude virus and dye, under any of the experimental conditions used, did not inactivate more than 1 to 2 logs of viral infectivity when held in the dark or when exposed to light during a period of 45 min. Complete inactivation of virus was achieved when clarified virus and dye were mixed and immediately exposed to the visible light source for 15 min. Prior incubation of clarified virus and dye permitted inactivation by methylene blue only, whereas no incubation prior to exposure resulted in three of the dyes contributing to inactivation. A concentration of 6 mug of neutral red, Toluidine Blue, methylene blue, and crystal violet was used per milliliter of virus suspension. Crystal violet was not a good viral inactivator under the conditions of the experimentation. Inactive virus induced the formation of neutralizing antibodies in adult chickens and mice. The antibody titer stimulated by the antigen treated with methylene blue and visible light was probably significant.
通过中性红、甲苯胺蓝和亚甲蓝与可见光的相互作用，研究了口蹄疫病毒的灭活情况。源自阿根廷的A型1株CANEFA口蹄疫病毒在组织培养中生长，并在粗制和澄清状态下进行测试。将病毒和染料混合，在黑暗中于4℃孵育45分钟，或者混合后不预先孵育直接暴露于可见光源。在任何实验条件下，粗制病毒与染料的混合物在黑暗中保存或在45分钟内暴露于光线下时，病毒感染力的灭活程度不超过1至2个对数。当澄清的病毒与染料混合并立即暴露于可见光源15分钟时，可实现病毒的完全灭活。预先孵育澄清的病毒与染料仅允许亚甲蓝实现灭活，而在暴露前不进行孵育则导致三种染料均有助于灭活。每毫升病毒悬液使用6微克的中性红、甲苯胺蓝、亚甲蓝和结晶紫。在实验条件下，结晶紫不是一种良好的病毒灭活剂。灭活的病毒在成年鸡和小鼠中诱导产生中和抗体。用亚甲蓝和可见光处理的抗原刺激产生的抗体效价可能具有显著意义。
