Kamel M Y, Anderson R L
J Bacteriol. 1966 Dec;92(6):1689-97. doi: 10.1128/jb.92.6.1689-1697.1966.
Kamel, M. Y. (Michigan State University, East Lansing), and R. L. Anderson. Metabolism of d-mannose in Aerobacter aerogenes: evidence for a cyclic pathway. J. Bacteriol. 92:1689-1697. 1966.-Evidence is presented which suggests a cyclic pathway for the constitutive utilization of d-mannose in extracts of Aerobacter aerogenes PRL-R3. d-Mannose is phosphorylated with d-glucose-6-phosphate to yield d-mannose-6-phosphate and d-glucose. d-Glucose-6-phosphate may be regenerated by isomerization of d-mannose-6-phosphate through d-fructose-6-phosphate, or by phosphorylation of d-glucose with adenosine-5'-triphosphate. The pathway involves the participation of four constitutive enzymes: d-glucose-6-phosphate isomerase, d-mannose-6-phosphate isomerase, a stereospecific d-glucokinase, and a phosphotransferase which phosphorylates d-mannose with d-glucose-6-phosphate, acetyl phosphate, or carbamyl phosphate. The absence of d-mannokinase (adenosine-5'-triphosphate:d-mannose phosphotransferase) activity in extracts of this organism suggests that the pathway may be of functional significance. Also, the pathway accounts for an apparent 2-epimerization of d-mannose to d-glucose that was observed in extracts.
卡迈勒，M. Y.（密歇根州立大学，东兰辛）和R. L. 安德森。产气气杆菌中D-甘露糖的代谢：循环途径的证据。《细菌学杂志》92:1689 - 1697。1966年。——本文提供的证据表明，产气气杆菌PRL - R3提取物中D-甘露糖的组成型利用存在一条循环途径。D-甘露糖与D-葡萄糖-6-磷酸发生磷酸化反应，生成D-甘露糖-6-磷酸和D-葡萄糖。D-葡萄糖-6-磷酸可通过D-甘露糖-6-磷酸经D-果糖-6-磷酸异构化再生，或通过D-葡萄糖与腺苷-5'-三磷酸磷酸化再生。该途径涉及四种组成型酶的参与：D-葡萄糖-6-磷酸异构酶、D-甘露糖-6-磷酸异构酶、一种立体特异性D-葡萄糖激酶以及一种用D-葡萄糖-6-磷酸、乙酰磷酸或氨甲酰磷酸使D-甘露糖磷酸化的磷酸转移酶。该生物体提取物中缺乏D-甘露糖激酶（腺苷-5'-三磷酸：D-甘露糖磷酸转移酶）活性，这表明该途径可能具有功能意义。此外，该途径解释了在提取物中观察到的D-甘露糖到D-葡萄糖明显的2-差向异构化现象。
