产气气杆菌中D-果糖的代谢：对缺乏D-果糖6-磷酸激酶和D-果糖1,6-二磷酸酶的突变体的分析。
Metabolism of D-fructose in Aerobacter aerogenes: analysis of mutants lacking D-fructose 6-phosphate kinase and D-fructose 1,6-diphosphatase.
作者信息
Sapico V, Hanson T E, Walter R W, Anderson R L
出版信息
J Bacteriol. 1968 Jul;96(1):51-4. doi: 10.1128/jb.96.1.51-54.1968.
Abstract
The relative significance of the pathways for the conversion of d-fructose to d-fructose 1,6-diphosphate via d-fructose 1-phosphate or d-fructose 6-phosphate in Aerobacter aerogenes PRL-R3 was assessed by observing growth patterns of mutants lacking either d-fructose 6-phosphate kinase or d-fructose 1,6-diphosphatase. The mutant lacking d-fructose 6-phosphate kinase grew well on d-fructose or glycerol but not on d-glucose, whereas the mutant lacking d-fructose 1,6-diphosphatase grew on d-glucose but not on d-fructose or glycerol. The data indicate that the pathway of d-fructose metabolism is primarily through d-fructose 1-phosphate rather than d-fructose 6-phosphate.
摘要
通过观察缺乏6-磷酸果糖激酶或1,6-二磷酸果糖酶的产气气杆菌PRL-R3突变体的生长模式，评估了在产气气杆菌PRL-R3中，经由1-磷酸果糖或6-磷酸果糖将D-果糖转化为1,6-二磷酸-D-果糖的途径的相对重要性。缺乏6-磷酸果糖激酶的突变体在D-果糖或甘油上生长良好，但在D-葡萄糖上不能生长，而缺乏1,6-二磷酸果糖酶的突变体在D-葡萄糖上生长，但在D-果糖或甘油上不能生长。数据表明，D-果糖代谢途径主要是通过1-磷酸果糖而不是6-磷酸果糖。
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