Kelker N E, Anderson R L
J Bacteriol. 1971 Jan;105(1):160-4. doi: 10.1128/jb.105.1.160-164.1971.
Sorbitol (d-glucitol) metabolism in Aerobacter aerogenes PRL-R3 is shown to proceed via the pathway: sorbitol --> sorbitol 6-phosphate --> d-fructose 6-phosphate. Sorbitol phosphorylation is mediated by a phosphoenolpyruvate (PEP):sorbitol 6-phosphotransferase system, and sorbitol 6-phosphate oxidation by a pyridine-nucleotide-linked dehydrogenase. Mutants deficient in sorbitol 6-phosphate dehydrogenase or a component (enzyme I) of the phosphotransferase system did not grow on sorbitol, whereas revertants which had regained these enzymatic activities grew normally. Extracts of the enzyme I-deficient mutant failed to catalyze the phosphorylation of sorbitol in the presence of PEP, and adenosine 5'-triphosphate could not replace the PEP requirement for sorbitol phosphorylation in extracts of the wild-type strain.
产气气杆菌PRL - R3中,山梨醇(d - 葡糖醇)的代谢途径为:山梨醇→6 - 磷酸山梨醇→6 - 磷酸果糖。山梨醇磷酸化由磷酸烯醇式丙酮酸(PEP):山梨醇6 - 磷酸转移酶系统介导,6 - 磷酸山梨醇氧化由吡啶核苷酸连接的脱氢酶介导。缺乏6 - 磷酸山梨醇脱氢酶或磷酸转移酶系统某一组分(酶I)的突变体不能在山梨醇上生长,而恢复了这些酶活性的回复体则能正常生长。缺乏酶I的突变体提取物在有PEP存在时不能催化山梨醇的磷酸化,并且腺苷5'-三磷酸不能替代野生型菌株提取物中山梨醇磷酸化对PEP的需求。