代谢抑制剂对厌氧培养酵母呼吸作用发展的影响。
The effect of metabolic inhibitors on the development of respiration in anaerobically grown yeast.
作者信息
Bartley W, Tustanoff E R
出版信息
Biochem J. 1966 Jun;99(3):599-603. doi: 10.1042/bj0990599.
Abstract
- Iodoacetate and fluoride did not prevent the development of respiration in aerobically grown yeast. 2. The effect of dinitrophenol suggested that phosphorylation developed simultaneously with respiration in anaerobically grown yeast, but the effect of oligomycin suggested that the phosphorylation and oxidation were not tightly coupled. 3. Inhibitors of electron transport showed that both the respiratory peak and the subsequent respiration were cyanide-sensitive, but the peak respiration was insensitive to antimycin. 4. Of the inhibitors of protein or RNA synthesis tested, only p-fluorophenylalanine inhibited the development of respiration. The results are not consistent with a new synthesis of mitochondria. 5. 2-Phenylethanol inhibited the development of respiration in anaerobically grown yeast and also yeast growth. Other inhibitors of DNA synthesis had no effect on the development of respiration. 6. The relevance of the results to mitochondrial morphogenesis is discussed.
摘要
- 碘乙酸盐和氟化物并不能阻止需氧生长酵母呼吸作用的发展。2. 二硝基苯酚的作用表明，厌氧生长酵母中磷酸化作用与呼吸作用同时发展，但寡霉素的作用表明磷酸化作用与氧化作用并非紧密偶联。3. 电子传递抑制剂表明，呼吸峰值及随后的呼吸作用均对氰化物敏感，但峰值呼吸作用对抗霉素不敏感。4. 在测试的蛋白质或RNA合成抑制剂中，只有对氟苯丙氨酸抑制呼吸作用的发展。这些结果与线粒体的新合成不一致。5. 2-苯乙醇抑制厌氧生长酵母的呼吸作用发展以及酵母生长。其他DNA合成抑制剂对呼吸作用发展没有影响。6. 讨论了这些结果与线粒体形态发生的相关性。
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