Kinosita K, Kawato S, Ikegami A
Biophys J. 1977 Dec;20(3):289-305. doi: 10.1016/S0006-3495(77)85550-1.
Decay of fluorescence polarization after an impulsive excitation is correlated with wobbling motion of fluorescent molecules in membranes. The motion is characterized by two parameters, a "wobbling diffusion constant" and a "degree of orientational constraint" both of which can be determined directly from experimentally obtained decay. Detailed discussion, including theoretically calculated time-courses of polarization decay, is given for several types of molecules embedded in lipid bilayers; these types cover a large part of fluorescent probes available at present. The theory is useful for the analysis of fluorescence polarization decay in any system where the orientation of fluorophore is restricted by the surrounding structure.
脉冲激发后荧光偏振的衰减与膜中荧光分子的摆动运动相关。该运动由两个参数表征，即“摆动扩散常数”和“取向约束程度”，这两个参数均可直接从实验获得的衰减中确定。文中针对嵌入脂质双层的几种类型的分子给出了详细讨论，包括偏振衰减的理论计算时间进程；这些类型涵盖了目前可用的大部分荧光探针。该理论对于分析荧光团取向受周围结构限制的任何系统中的荧光偏振衰减都很有用。
