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哺乳动物胰腺中磷脂酸和磷脂酰肌醇的生物合成。

The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas.

作者信息

Prottey C, Hawthorne J N

出版信息

Biochem J. 1967 Oct;105(1):379-92. doi: 10.1042/bj1050379.

DOI:10.1042/bj1050379
PMID:4293959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1198310/
Abstract
  1. The labelling of guinea-pig pancreas phospholipids in vivo after intraperitoneal injection of [(32)P]orthophosphate is described. 2. Acyl-CoA synthetase activity in pancreas homogenates has been studied. There is no absolute requirement for added fatty acids, indicating an adequate supply of endogenous fatty acids in these preparations. 3. Phosphatidic acid is formed in guinea-pig pancreas preparations by two distinct routes, namely the acylation of l-3-glycerophosphate and the phosphorylation of 1,2-diglyceride. Phosphatidic acid formed by either mechanism is converted into phosphatidylinositol by guinea-pig pancreas in vitro. 4. The enzymes of pancreas that convert phosphatidic acid into phosphatidylinositol via CDP-diglyceride have been characterized. 5. Addition of bovine serum albumin is necessary in assaying certain of these enzymes.
摘要
  1. 描述了腹腔注射[（32）P]正磷酸盐后豚鼠胰腺磷脂在体内的标记情况。2. 对胰腺匀浆中的酰基辅酶A合成酶活性进行了研究。这些制剂中对添加脂肪酸没有绝对需求，这表明内源性脂肪酸供应充足。3. 豚鼠胰腺制剂中磷脂酸通过两条不同途径形成，即1-3-甘油磷酸的酰化和1,2-二甘油酯的磷酸化。通过任何一种机制形成的磷脂酸在体外都可被豚鼠胰腺转化为磷脂酰肌醇。4. 已经对胰腺中通过CDP-二甘油酯将磷脂酸转化为磷脂酰肌醇的酶进行了表征。5. 在测定其中某些酶时，添加牛血清白蛋白是必要的。

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本文引用的文献

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Biochem J. 1932;26(2):292-7. doi: 10.1042/bj0260292.
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Respiration and phosphorylation in preparations from Mammalian Brain.哺乳动物脑提取物中的呼吸作用与磷酸化作用。
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METABOLISM OF GLYCEROLIPIDS. VI. SPECIFICITIES OF ACYL COENZYME A: PHOSPHOLIPID ACYLTRANSFERASES.甘油脂质的代谢。VI. 酰基辅酶A：磷脂酰基转移酶的特异性。
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SPECIFIC ASSAYS OF SOME PHOSPHATASES IN SUBCELLULAR FRACTIONS OF SMALL INTESTINAL MUCOSA.小肠黏膜亚细胞组分中某些磷酸酶的特异性测定
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IONIC PROPERTIES OF AQUEOUS DISPERSIONS OF PHOSPHATIDIC ACID.磷脂酸水分散体的离子特性
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THE ENZYMATIC ACYLATION OF LYSOPHOSPHATIDYLINOSITOL.溶血磷脂酰肌醇的酶促酰化作用
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Assay of glycerol phosphate acyltransferase in liver particles.肝脏微粒体中甘油磷酸酰基转移酶的测定
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Biosynthesis of phosphatidylinositol in rat brain.大鼠脑中磷脂酰肌醇的生物合成
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