粟酒裂殖酵母中的磷脂酸和磷脂酰肌醇代谢
Phosphatidic acid and phosphatidylinositol metabolism in Schizosaccharomyces pombe.
作者信息
White G L, Hawthorne J N
出版信息
Biochem J. 1970 Apr;117(2):203-13. doi: 10.1042/bj1170203.
Abstract
The phospholipid composition of Schizosaccharomyces pombe was not markedly affected by changes in the phosphate concentration of the medium or phase of growth. The major fatty acids in the total lipid extract and purified phosphatidylinositol were palmitic acid and oleic acid. Phosphatidic acid was synthesized by acylation of l-3-glycerophosphate in Schiz. pombe and phosphatidate phosphohydrolase was present. Phosphatidylinositol synthesis from inositol occurred in the absence of CDP-diglyceride. Even with dialysed cell-free preparations, the inositol lipid was synthesized by an apparently energy-independent route, at rates greater than would be required during cell growth. Phosphatidylinositol appeared to be broken down by a phospholipase D. All the enzymes examined were particulate; similar activities were found in Saccharomyces cerevisiae.
摘要
粟酒裂殖酵母的磷脂组成并未受到培养基中磷酸盐浓度变化或生长阶段的显著影响。总脂质提取物和纯化的磷脂酰肌醇中的主要脂肪酸为棕榈酸和油酸。在粟酒裂殖酵母中，磷脂酸通过1,3-甘油磷酸的酰化作用合成，且存在磷脂酸磷酸水解酶。在没有CDP-二甘油酯的情况下，由肌醇合成磷脂酰肌醇。即使是经过透析的无细胞制剂，肌醇脂质也通过一条明显不依赖能量的途径合成，其合成速率高于细胞生长所需的速率。磷脂酰肌醇似乎被磷脂酶D分解。所有检测的酶都是颗粒性的；在酿酒酵母中也发现了类似的活性。
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