鸡胚绒毛尿囊膜细胞的体外培养。
In vitro cultivation of cells from the chorioallantoic membrane of chick embryos.
作者信息
Cursiefen D, Becht H
出版信息
Med Microbiol Immunol. 1975;161(1):3-10. doi: 10.1007/BF02120764.
Abstract
By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.
摘要
在用胰蛋白酶常规处理之前，先用胶原酶和透明质酸酶处理鸡胚绒毛尿囊膜，这样培养的细胞能够支持多种黏液病毒、疱疹病毒、禽呼肠孤病毒和鸡传染性支气管炎病毒的生长。这些培养物可用于敏感的蚀斑测定和中和试验。
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引用本文的文献
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The site of cleavage in infected cells and polypeptides of representative paramyxoviruses grown in cultured cells of the chorioallantoic membrane.感染细胞中的裂解位点以及在绒毛尿囊膜培养细胞中生长的代表性副粘病毒的多肽。
Arch Virol. 1981;67(1):19-30. doi: 10.1007/BF01314598.
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Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses.对感染代表性副粘病毒的绒毛尿囊膜培养细胞进行的电子显微镜研究。
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Structural and growth characteristics of two avian reoviruses.两种禽呼肠孤病毒的结构与生长特性
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本文引用的文献
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THE CULTIVATION OF VACCINE AND OTHER VIRUSES IN THE CHORIOALLANTOIC MEMBRANE OF CHICK EMBRYOS.疫苗及其他病毒在鸡胚绒毛尿囊膜中的培养
Science. 1931 Oct 9;74(1919):371-2. doi: 10.1126/science.74.1919.371.
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Cultivation of influenza virus in the chorio-allantoic membrane of deembryonated eggs.在去胚鸡蛋的绒毛尿囊膜上培养流感病毒。
Proc Soc Exp Biol Med. 1949 Dec;72(3):680-2. doi: 10.3181/00379727-72-17541.
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Observations with the electron microscope on cells of the chick chorio-allantoic membrane infected with influenza virus.用电子显微镜对感染流感病毒的鸡绒毛尿囊膜细胞进行的观察。
J Exp Med. 1952 Mar;95(3):259-68. doi: 10.1084/jem.95.3.259.
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Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus.病毒-多糖相互作用。I. 一种决定脑心肌炎病毒噬菌斑形态的琼脂多糖。
Virology. 1961 Aug;14:456-62. doi: 10.1016/0042-6822(61)90338-5.
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Propagation of duck hepatitis virus in tissue cultures prepared with collagenase.鸭肝炎病毒在经胶原酶处理的组织培养物中的增殖
J Bacteriol. 1960 Oct;80(4):571-2. doi: 10.1128/jb.80.4.571-572.1960.
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Physical and biological properties of influenza virus components obtained after ether treatment.经乙醚处理后获得的流感病毒成分的物理和生物学特性。
J Exp Med. 1960 Nov 1;112(5):765-82. doi: 10.1084/jem.112.5.765.
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Mammalian cell cultures for study of influenza virus. I. Preparation of monolayer cultures with collagenase.用于流感病毒研究的哺乳动物细胞培养。I. 用胶原酶制备单层培养物
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J Hyg (Lond). 1958 Dec;56(4):535-46. doi: 10.1017/s002217240003802x.
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Quantitative studies on Rous sarcoma virus. III Virus multiplication and cellular response following infection of the chorioallantoic membrane of the chick embryo.劳斯肉瘤病毒的定量研究。III. 鸡胚绒毛尿囊膜感染后的病毒增殖和细胞反应
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