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鸡胚绒毛尿囊膜细胞的体外培养。

In vitro cultivation of cells from the chorioallantoic membrane of chick embryos.

作者信息

Cursiefen D, Becht H

出版信息

Med Microbiol Immunol. 1975;161(1):3-10. doi: 10.1007/BF02120764.

DOI:10.1007/BF02120764
PMID:165393
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7087208/
Abstract

By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.

摘要

在用胰蛋白酶常规处理之前，先用胶原酶和透明质酸酶处理鸡胚绒毛尿囊膜，这样培养的细胞能够支持多种黏液病毒、疱疹病毒、禽呼肠孤病毒和鸡传染性支气管炎病毒的生长。这些培养物可用于敏感的蚀斑测定和中和试验。

相似文献

1
In vitro cultivation of cells from the chorioallantoic membrane of chick embryos.鸡胚绒毛尿囊膜细胞的体外培养。
Med Microbiol Immunol. 1975;161(1):3-10. doi: 10.1007/BF02120764.
2
The growth cycle of influenza as studied in touch preparations of the allantoic layer of chick embryos. 1. The number of cells lining the allantoic cavity.在鸡胚尿囊层涂片制备物中研究的流感生长周期。1. 尿囊腔内衬细胞的数量。
Zentralbl Bakteriol Orig A. 1975 May;231(4):393-420.
3
A new method for propagation of HVJ (Sendai virus) in vitro: high sensitivity and productivity in spreading cultures of fragmented chorioallantoic membrane of chick embryos.一种在体外繁殖HVJ（仙台病毒）的新方法：在鸡胚绒毛尿囊膜碎片的铺展培养物中具有高敏感性和高产量。
Biken J. 1974 Jun;17(2):51-8.
4
[Replication characteristics and cytopathic effect of southern German field isolates of chicken infectious bronchitis virus in cell cultures and the effect of trypsin].[德国南部鸡传染性支气管炎病毒野毒株在细胞培养中的复制特性、细胞病变效应及胰蛋白酶的作用]
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5
Investigations concerning the cultivation of myxo- and paramyxoviruses on chorioallantoic membrane fragments. Note I. Data on the multiplication of several myxo- and paramyxoviruses.关于黏液病毒和副黏液病毒在绒毛尿囊膜碎片上培养的研究。注释I. 几种黏液病毒和副黏液病毒增殖的数据。
Virologie. 1981 Apr-Jun;32(2):145-54.
6
Autoradiographic demonstration of target cells for 1,25-dihydroxycholecalciferol in the chick embryo chorioallantoic membrane, duodenum, and parathyroid glands.1,25 - 二羟胆钙化醇在鸡胚绒毛尿囊膜、十二指肠和甲状旁腺中靶细胞的放射自显影证明。
Gen Comp Endocrinol. 1980 Nov;42(3):283-9. doi: 10.1016/0016-6480(80)90156-2.
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Plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin.在胰蛋白酶存在的情况下，禽传染性支气管炎病毒在原代鸡胚成纤维细胞中形成斑块。
Arch Virol. 1981;70(4):315-20. doi: 10.1007/BF01320246.
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Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses.对感染代表性副粘病毒的绒毛尿囊膜培养细胞进行的电子显微镜研究。
Arch Virol. 1980;65(3-4):247-55. doi: 10.1007/BF01314541.
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The site of cleavage in infected cells and polypeptides of representative paramyxoviruses grown in cultured cells of the chorioallantoic membrane.感染细胞中的裂解位点以及在绒毛尿囊膜培养细胞中生长的代表性副粘病毒的多肽。
Arch Virol. 1981;67(1):19-30. doi: 10.1007/BF01314598.
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[Cell cycle parameters and proliferative activity in chorioallantois tissues at various stages in chick embryo development].[鸡胚发育各阶段绒毛尿囊膜组织中的细胞周期参数与增殖活性]
Tsitologiia. 1985 Jun;27(6):720-5.

引用本文的文献

1
The site of cleavage in infected cells and polypeptides of representative paramyxoviruses grown in cultured cells of the chorioallantoic membrane.感染细胞中的裂解位点以及在绒毛尿囊膜培养细胞中生长的代表性副粘病毒的多肽。
Arch Virol. 1981;67(1):19-30. doi: 10.1007/BF01314598.
2
Acylation of viral spike glycoproteins: a feature of enveloped RNA viruses.病毒刺突糖蛋白的酰化：包膜RNA病毒的一个特征。
Virology. 1982 Jan 15;116(1):327-38. doi: 10.1016/0042-6822(82)90424-x.
3
Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses.对感染代表性副粘病毒的绒毛尿囊膜培养细胞进行的电子显微镜研究。
Arch Virol. 1980;65(3-4):247-55. doi: 10.1007/BF01314541.
4
Collagenase in equine cell culture preparation.马细胞培养制备中的胶原酶。
J Clin Microbiol. 1979 Jun;9(6):731-3. doi: 10.1128/jcm.9.6.731-733.1979.
5
Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose.副粘病毒感染会刺激细胞多肽的合成，而在劳斯肉瘤病毒转化的细胞或缺乏葡萄糖的细胞中也会出现这种刺激现象。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6120-4. doi: 10.1073/pnas.75.12.6120.
6
Structural and growth characteristics of infectious bursal disease virus.传染性法氏囊病病毒的结构与生长特性
J Virol. 1976 Apr;18(1):227-34. doi: 10.1128/JVI.18.1.227-234.1976.
7
Structural and growth characteristics of two avian reoviruses.两种禽呼肠孤病毒的结构与生长特性
Arch Virol. 1975;48(3):261-9. doi: 10.1007/BF01317969.

本文引用的文献

1
THE CULTIVATION OF VACCINE AND OTHER VIRUSES IN THE CHORIOALLANTOIC MEMBRANE OF CHICK EMBRYOS.疫苗及其他病毒在鸡胚绒毛尿囊膜中的培养
Science. 1931 Oct 9;74(1919):371-2. doi: 10.1126/science.74.1919.371.
2
Cultivation of influenza virus in the chorio-allantoic membrane of deembryonated eggs.在去胚鸡蛋的绒毛尿囊膜上培养流感病毒。
Proc Soc Exp Biol Med. 1949 Dec;72(3):680-2. doi: 10.3181/00379727-72-17541.
3
Observations with the electron microscope on cells of the chick chorio-allantoic membrane infected with influenza virus.用电子显微镜对感染流感病毒的鸡绒毛尿囊膜细胞进行的观察。
J Exp Med. 1952 Mar;95(3):259-68. doi: 10.1084/jem.95.3.259.
4
Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus.病毒-多糖相互作用。I. 一种决定脑心肌炎病毒噬菌斑形态的琼脂多糖。
Virology. 1961 Aug;14:456-62. doi: 10.1016/0042-6822(61)90338-5.
5
Propagation of duck hepatitis virus in tissue cultures prepared with collagenase.鸭肝炎病毒在经胶原酶处理的组织培养物中的增殖
J Bacteriol. 1960 Oct;80(4):571-2. doi: 10.1128/jb.80.4.571-572.1960.
6
Physical and biological properties of influenza virus components obtained after ether treatment.经乙醚处理后获得的流感病毒成分的物理和生物学特性。
J Exp Med. 1960 Nov 1;112(5):765-82. doi: 10.1084/jem.112.5.765.
7
Mammalian cell cultures for study of influenza virus. I. Preparation of monolayer cultures with collagenase.用于流感病毒研究的哺乳动物细胞培养。I. 用胶原酶制备单层培养物
Proc Soc Exp Biol Med. 1959 May;101(1):19-22. doi: 10.3181/00379727-101-24818.
8
Comparison of the infectivity of influenza viruses in two host systems: the allantois of intact eggs and surviving allantois-on-shell.两种宿主系统中流感病毒感染性的比较：完整鸡蛋的尿囊和存活的带壳尿囊。
J Hyg (Lond). 1958 Dec;56(4):535-46. doi: 10.1017/s002217240003802x.
9
Quantitative studies on Rous sarcoma virus. III Virus multiplication and cellular response following infection of the chorioallantoic membrane of the chick embryo.劳斯肉瘤病毒的定量研究。III. 鸡胚绒毛尿囊膜感染后的病毒增殖和细胞反应
Virology. 1958 Jun;5(3):435-57. doi: 10.1016/0042-6822(58)90038-2.
10
Inclusion body formation with Newcastle disease and mumps viruses in cultures of chick embryo cells.鸡胚细胞培养物中新城疫病毒和腮腺炎病毒的包涵体形成。
Virology. 1958 Apr;5(2):177-91. doi: 10.1016/0042-6822(58)90017-5.