豌豆线粒体中烟酰胺腺嘌呤二核苷酸特异性异柠檬酸脱氢酶。纯化及特性
Nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Purification and properties.
作者信息
Cox G F, Davies D D
出版信息
Biochem J. 1967 Nov;105(2):729-34. doi: 10.1042/bj1050729.
Abstract
- A method of stabilizing the enzyme by using glycerol is described. 2. A purification procedure is presented giving a higher purification than previously described. 3. Data showing substrate activation and activation by citrate are presented. 4. Kinetic constants for NAD(+), NADH and certain bivalent metal ions are given. 5. Pronounced inhibitory buffer effects are described. 6. A brief comparison between the NAD-specific isocitrate dehydrogenase from peas and that from other sources is made.
摘要
- 描述了一种使用甘油稳定酶的方法。2. 提出了一种纯化程序，其纯化程度高于先前所述。3. 给出了显示底物激活和柠檬酸激活的数据。4. 给出了NAD(+)、NADH和某些二价金属离子的动力学常数。5. 描述了显著的缓冲抑制作用。6. 对豌豆中的NAD特异性异柠檬酸脱氢酶与其他来源的该酶进行了简要比较。
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