pH值和柠檬酸盐对豌豆线粒体中烟酰胺腺嘌呤二核苷酸特异性异柠檬酸脱氢酶活性的影响。
The effects of pH and citrate on the activity of nicotinamideA-denine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria.
作者信息
Cox G F, Davies D D
出版信息
Biochem J. 1969 Aug;113(5):813-20. doi: 10.1042/bj1130813.
Abstract
- The effect of pH on the co-operative activation of the NAD-specific isocitrate dehydrogenase from pea mitochondria by isocitrate is shown. 2. The interlinked effects of pH on the affinity of the NAD-specific isocitrate dehydrogenase for isocitrate and the dependence of the pH optimum on the substrate concentration are presented. 3. A consideration of the conditions of pH and substrate concentration under which citrate activates the NAD-specific isocitrate dehydrogenase demonstrates similarities between the binding of isocitrate and citrate. 4. A comparison of the effects of citrate and pH on the gross structure of the enzyme is investigated by density-gradient centrifugation. 5. The kinetic interpretations of these results are briefly considered. 6. The metabolic significance of these studies is discussed.
摘要
- 显示了pH对豌豆线粒体中NAD特异性异柠檬酸脱氢酶由异柠檬酸协同激活的影响。2. 阐述了pH对NAD特异性异柠檬酸脱氢酶与异柠檬酸亲和力的相互关联影响以及最适pH对底物浓度的依赖性。3. 对pH和底物浓度条件下柠檬酸盐激活NAD特异性异柠檬酸脱氢酶的情况进行考量，揭示了异柠檬酸和柠檬酸盐结合之间的相似性。4. 通过密度梯度离心研究了柠檬酸盐和pH对酶整体结构影响的比较。5. 简要考虑了这些结果的动力学解释。6. 讨论了这些研究的代谢意义。
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本文引用的文献
1
The Combinations of Haemoglobin with Oxygen and with Carbon Monoxide. I.血红蛋白与氧气及一氧化碳的结合。I.
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2
3
4
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J Biol Chem. 1964 May;239:1597-603.
5
ACTIVATION AND INHIBITION OF DPN-LINKED ISOCITRATE DEHYDROGENASE OF HEART BY CERTAIN NUCLEOTIDES.某些核苷酸对心脏中与二磷酸硫胺素相关的异柠檬酸脱氢酶的激活与抑制作用
Biochemistry. 1963 Sep-Oct;2:1023-32. doi: 10.1021/bi00905a020.
6
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Biochem Biophys Res Commun. 1963 Aug 20;12:510-5. doi: 10.1016/0006-291x(63)90325-5.
7
8
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
9
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