pH对豌豆线粒体中烟酰胺腺嘌呤二核苷酸特异性异柠檬酸脱氢酶与烟酰胺腺嘌呤二核苷酸结合特性的影响。
The effect of pH on the characteristics of the binding of nicotinamide-adenine dinucleotide by nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria.
作者信息
Cox G F, Davies D D
出版信息
Biochem J. 1970 Mar;116(5):819-24. doi: 10.1042/bj1160819.
Abstract
- The effect of pH on the V(max.) and concentration of NAD(+) at half-maximum velocity at a constant isocitrate concentration was examined, and the results were related to the requirements for binding of H(+) ions to the enzyme. 2. The effect of varying the NAD(+) concentration on the pH optimum with constant isocitrate concentration was studied. 3. A comparison has been made between the effect of isocitrate concentration on the characteristics of binding of NAD(+) and the effect of NAD(+) concentration on the characteristics of isocitrate binding at three different pH values. 4. The mechanistic and metabolic significance of these studies is considered.
摘要
- 在异柠檬酸浓度恒定的情况下，研究了pH对最大反应速度（V(max.)）以及达到最大反应速度一半时NAD(+)浓度的影响，并将结果与H(+)离子与酶结合的需求相关联。2. 在异柠檬酸浓度恒定的情况下，研究了改变NAD(+)浓度对最适pH的影响。3. 比较了在三种不同pH值下，异柠檬酸浓度对NAD(+)结合特性的影响以及NAD(+)浓度对异柠檬酸结合特性的影响。4. 考虑了这些研究的机制和代谢意义。
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