Maughan R J
Clin Chim Acta. 1982 Jul 1;122(2):231-40. doi: 10.1016/0009-8981(82)90282-0.
Existing methods for the estimation of glucose, lactate, pyruvate, alanine, 3-hydroxybutyrate and acetoacetate normally require relatively large volumes of blood. In addition spectrophotometric methods are tedious and time-consuming. A series of simple methods for the measurement of these metabolites whereby all can be measured on a single 20-microl blood sample is described here. All methods, with the exception of that for glucose, which is measured spectrophotometrically, depend on the use of fluorimetry to determine the concentration of the reduced co-enzyme NADH. As far as is possible, the methods have been standardised for the sake of simplicity and convenience. In routine use they have proved to be both rapid and reliable.
现有的用于估算葡萄糖、乳酸、丙酮酸、丙氨酸、3-羟基丁酸和乙酰乙酸的方法通常需要相对大量的血液。此外，分光光度法既繁琐又耗时。本文介绍了一系列用于测量这些代谢物的简单方法，通过这些方法，所有代谢物都可以在一份20微升的血液样本上进行测量。除了用分光光度法测量葡萄糖的方法外，所有方法都依赖于使用荧光法来测定还原型辅酶NADH的浓度。为了简单和方便起见，这些方法已尽可能实现标准化。在常规使用中，它们已被证明既快速又可靠。
