Bolognesi D P, Langlois A J, Sverak L, Bonar R A, Beard J W
J Virol. 1968 Jun;2(6):576-86. doi: 10.1128/JVI.2.6.576-586.1968.
Strain MC29 avian leukosis (myelocytomatosis) virus induced infection, elaboration of virus, and morphological alteration in chick embryo cells in vitro. Virus liberation began within 18 hr, morphological change was detectable at about 40 hr, and the cultures could be completely altered within 80 hr after infection. Altered cells were about half the volume and grew at approximately twice the rate of uninfected elements. The output of virus estimated by electron microscopy was about 140 particles per cell per hr. Deoxyribonucleic acid remained constant, but ribonucleic acid increased in both infected and control cells in adjustment to culture environment. The rates of uptake and incorporation of (3)H-uridine and the incorporation of (3)H-thymidine increased in the infected cells with onset of morphological change but were unaffected by processes of infection and virus elaboration per se. Incorporation of a (14)C-amino acid mixture was slightly greater in the infected than in control cells. The speed of continuity of infection and massive morphological alteration constitute a unique response to avian tumor viruses, and the system gives promise of singular value for detailed studies of the processes of infection and morphological change.
MC29株禽白血病（骨髓细胞瘤病）病毒在体外可引起鸡胚细胞感染、病毒增殖及形态学改变。病毒释放于18小时内开始，约40小时可检测到形态变化，感染后80小时内培养物可完全改变。改变后的细胞体积约为未感染细胞的一半，生长速度约为未感染细胞的两倍。通过电子显微镜估计，每细胞每小时产生的病毒颗粒约为140个。脱氧核糖核酸保持恒定，但核糖核酸在感染细胞和对照细胞中均因适应培养环境而增加。随着形态变化的出现，感染细胞中（3）H-尿苷的摄取和掺入率以及（3）H-胸苷的掺入率增加，但感染和病毒增殖过程本身对其无影响。感染细胞中（14）C-氨基酸混合物的掺入略高于对照细胞。感染的连续性和大规模形态学改变的速度构成了对禽肿瘤病毒的独特反应，该系统有望为详细研究感染过程和形态变化提供独特价值。
