与MC29菌株肿瘤病毒相关的脱氧核糖核酸聚合酶活性。
Deoxyribonucleic acid polymerase activity associated with strain MC29 tumor virus.
作者信息
Weber G H, Kiessling A A, Beaudreau G S
出版信息
J Virol. 1971 Feb;7(2):214-20. doi: 10.1128/JVI.7.2.214-220.1971.
Abstract
Chick embryo cells infected with strain MC29 tumor virus yielded progeny virus that contained detectable deoxyribonucleic acid (DNA) polymerase within the first 48 hr after infection. The noninfected culture fluids displayed no such enzyme activity when examined in an identical manner. Enzyme activity was greatly stimulated by adding DNA template to the reaction mixture and required all four deoxyribonucleoside triphosphates for full activity. When calf thymus DNA was used to direct synthesis, the DNA polymerase from the MC29 virus catalyzed the formation of DNA product having a higher buoyant density in CsCl. DNA product formed in the reaction directed by Micrococcus lysodeikticus DNA had the same buoyant density as the template DNA.
摘要
感染MC29肿瘤病毒的鸡胚细胞在感染后的头48小时内产生了子代病毒，该子代病毒含有可检测到的脱氧核糖核酸（DNA）聚合酶。以相同方式检测时，未感染的培养液没有这种酶活性。通过向反应混合物中添加DNA模板，酶活性得到极大刺激，并且完全活性需要所有四种脱氧核苷三磷酸。当使用小牛胸腺DNA来指导合成时，来自MC29病毒的DNA聚合酶催化形成在CsCl中具有更高浮力密度的DNA产物。在溶壁微球菌DNA指导的反应中形成的DNA产物具有与模板DNA相同的浮力密度。
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