The distribution and mobility of surface anionic groups of normal human oesophageal epithelium following interaction with cationized verritin.

Oesophageal epithelial cells from biopsies from normal patients showed the presence of randomly distributed anionic groups, mostly sialic acid on the cell membrane in fixed material shown by cationized ferritin. When biopsies were pulse labelled, patching occurred in all three cell layers. Patching was energy dependent and did not occur at 4 degrees C. Pulse labelled material incubated on an unlabelled medium showed progressive loss of cationized ferritin from the cell membrane. This was mostly into the medium, although some was internalized in membrane profiles. A second pulse of cationized ferritin produced further patching suggesting regeneration of cell membrane. Superficial cells were leaky, but their organelles were not.