Hopwood D, Logan K R, Bouchier I A
Virchows Arch B Cell Pathol. 1978 Feb 14;26(4):345-58. doi: 10.1007/BF02889561.
Oesophageal biopsies were studied with the electron microscope. Three layers were identified, as in the light microscopy of the oesophageal epithelium: basal, prickle and funtional cell layers. A continuous basement membrane separated the lamina propria from the basal cells. The basal cell membrane carried hemidesmosomes, desmosomes and microvillous processes. Their cytoplasm contained the usual organelles plus free ribosomes and tonofirbrils. Prickle cells contained glycogen rosettes and many tonofilaments, and their cell membrane many microvillous and demosomal processes, in places elaborated into desmosome fields. In both these layers there was a wide intercellular space containing some particulate and membranous debris. The flattened cells of the functional layer had fewer desmosomes and microvilli but abundant glycogen and tonofilaments, and a narrow intercellular space. Membrane coating granules first reaching a maximum in the functional cell layer appeared in the upper prickle cell layer and few persisted into the surface cells. The apical cell membrane of the most superficial cells was thickened and had few small microvillous processes, which were covered with a filamentous "fuzzy" coat. No keratohyaline granules were present. Papillae of lamina propria contained capillaries, some with a fenestrated endothelium.
对食管活检组织进行了电子显微镜研究。与食管上皮的光学显微镜观察结果一样，可识别出三层：基底细胞层、棘细胞层和功能细胞层。一层连续的基底膜将固有层与基底细胞分隔开来。基底细胞膜上有半桥粒、桥粒和微绒毛突起。其细胞质中含有常见的细胞器以及游离核糖体和张力丝。棘细胞含有糖原玫瑰花样结构和许多张力丝，其细胞膜有许多微绒毛和桥粒样突起，在某些部位形成桥粒斑。在这两层中都有一个宽大的细胞间隙，其中含有一些颗粒状和膜状碎片。功能层的扁平细胞桥粒和微绒毛较少，但糖原和张力丝丰富，细胞间隙狭窄。膜被颗粒首先在上层棘细胞层达到最大值，出现在上层棘细胞层，很少持续存在于表面细胞中。最表层细胞的顶端细胞膜增厚，有少量小的微绒毛突起，这些突起覆盖着丝状的“模糊”外衣。不存在透明角质颗粒。固有层乳头含有毛细血管，有些毛细血管内皮有窗孔。
