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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

J Exp Med. 1969 Mar 1;129(3):523-50. doi: 10.1084/jem.129.3.523.

Leukocytosis- and lymphocytosis-stimulating activity was present in fluid cultures of B. pertussis. The activity was found primarily in the culture supernatant fluid. 2. The sequential changes in the leukocyte response were similar to those previously observed following injection of intact bacteria into mice. 3. Activity was destroyed by heat and was diminished, but not abolished, by prolonged treatment with proteolytic enzymes. 4. A water-insoluble fraction of the culture supernatant fluid was isolated which contained virtually all of the activity. The specific activity was more than 100-fold greater than that of the intact bacteria, and injection of microgram quantities produced a response. 5. The distribution of histamine-sensitizing factor followed that of leukocytosis-stimulating activity. In contrast, mouse protective antigen was localized to the bacterial pellet.

百日咳博德特氏菌的液体培养物中存在刺激白细胞增多和淋巴细胞增多的活性。该活性主要存在于培养上清液中。2. 白细胞反应的顺序变化与先前将完整细菌注射到小鼠体内后观察到的变化相似。3. 活性被加热破坏，经蛋白水解酶长时间处理后活性降低但未消除。4. 分离出培养上清液的水不溶性部分，其几乎包含所有活性。比活性比完整细菌高100倍以上，注射微克量即可产生反应。5. 组胺致敏因子的分布与刺激白细胞增多活性的分布一致。相比之下，小鼠保护性抗原定位于细菌沉淀中。

J Exp Med. 1969 Mar 1;129(3):523-50. doi: 10.1084/jem.129.3.523.

Leukocytosis- and lymphocytosis-stimulating activity was present in fluid cultures of B. pertussis. The activity was found primarily in the culture supernatant fluid. 2. The sequential changes in the leukocyte response were similar to those previously observed following injection of intact bacteria into mice. 3. Activity was destroyed by heat and was diminished, but not abolished, by prolonged treatment with proteolytic enzymes. 4. A water-insoluble fraction of the culture supernatant fluid was isolated which contained virtually all of the activity. The specific activity was more than 100-fold greater than that of the intact bacteria, and injection of microgram quantities produced a response. 5. The distribution of histamine-sensitizing factor followed that of leukocytosis-stimulating activity. In contrast, mouse protective antigen was localized to the bacterial pellet.

百日咳博德特氏菌的液体培养物中存在刺激白细胞增多和淋巴细胞增多的活性。该活性主要存在于培养上清液中。2. 白细胞反应的顺序变化与先前将完整细菌注射到小鼠体内后观察到的变化相似。3. 活性被加热破坏，经蛋白水解酶长时间处理后活性降低但未消除。4. 分离出培养上清液的水不溶性部分，其几乎包含所有活性。比活性比完整细菌高100倍以上，注射微克量即可产生反应。5. 组胺致敏因子的分布与刺激白细胞增多活性的分布一致。相比之下，小鼠保护性抗原定位于细菌沉淀中。