Desiatskova R G
Vopr Virusol. 1977 Mar-Apr(2):178-83.
The plaque-forming properties of 8 freshly isolated, 2 attenuated strains, RA 27/3 and Cendehill, and Sp-2 strain adapted to BHK-21/13C cell culture were studied. The concentration of sodium bicarbonate in the agar overlay influenced significantly the plaque production by rubella virus. Its optimum concentration was 0.22%. At higher (0.45%) and very low (0.11%) concentrations of sodium bicarbonate all the strains produced a smaller number of plaques. The strains were found to be sensitive to inhibitors in agar, the sensitivity being manifested in a smaller size of the plaques and reduction in their number. The strains were found to differ in the size and pattern of the plaques produced. Both among fresh isolates and attenuated strains, large-plaque (S+), small-plaque (S-) and S+/- strains were found. The S marker in combination with other markers may be used for differentiation of rubella virus strains.
研究了8株新分离株、2株减毒株（RA 27/3和森德希尔株）以及适应BHK - 21/13C细胞培养的Sp - 2株的蚀斑形成特性。琼脂覆盖层中碳酸氢钠的浓度对风疹病毒蚀斑产生有显著影响。其最佳浓度为0.22%。在较高（0.45%）和极低（0.11%）浓度的碳酸氢钠条件下，所有毒株产生的蚀斑数量均较少。发现这些毒株对琼脂中的抑制剂敏感，敏感性表现为蚀斑尺寸变小和数量减少。发现这些毒株产生的蚀斑在大小和形态上存在差异。在新鲜分离株和减毒株中均发现了大蚀斑（S +）、小蚀斑（S -）和S + / - 毒株。S标记与其他标记结合可用于风疹病毒株的鉴别。
