通过噬菌体λ的红色功能实现噬菌体φX174的重组。
Recombination of bacteriophage phi X174 by the red function of bacteriophage lambda.
作者信息
Munekiyo R, Sekiguchi M
出版信息
J Virol. 1979 Feb;29(2):438-42. doi: 10.1128/JVI.29.2.438-442.1979.
Abstract
Recombination of bacteriophage phi X174 was effectively promoted when the Red function of lambda was supplied by either co-infection with lambda or induction of lambda lysogens. Mutations in red alpha and red beta genes of lambda abolished recombination nearly completely, whereas a mutation in gam gene reduced it only slightly. The Red-promoted recombination of phi X174 occurred in recA, recB, and polA mutants as well as in wild-type strains of Escherichia coli. It was further stimulated when phi X174 mutants were irradiated with UV light before infection.
摘要
当通过与λ共感染或诱导λ溶原菌来提供λ的Red功能时，噬菌体φX174的重组得到有效促进。λ的redα和redβ基因中的突变几乎完全消除了重组，而gam基因中的突变仅使其略有减少。φX174的Red促进的重组发生在recA、recB和polA突变体以及大肠杆菌的野生型菌株中。当φX174突变体在感染前用紫外线照射时，重组进一步受到刺激。
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