λ噬菌体诱导所需紫外线剂量与λ阻遏物浓度之间的相关性。
Correlation between UV dose requirement for lambda bacteriophage induction and lambda repressor concentration.
作者信息
Baluch J, Sussman R
出版信息
J Virol. 1978 Jun;26(3):595-602. doi: 10.1128/JVI.26.3.595-602.1978.
Abstract
Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentration of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to UV irradiation were determined. In both strains, dose-response relationships were obtained as a function of the cellular repressor concentration. The uvrA lysogens required one-tenth the UV fluence of the wild-type counterparts for induction. Lysogenic strains containing plasmids that overproduce the lambdaind+ repressor and the same lysogens with plasmids overproducing the lambdaind- repressor displayed the same survival curves as the nonlysogenic parental strain; however, only the former produced infectious centers (at a frequency of 2 x 10(-3) to 5 x 10(-4) in response to radiation.
摘要
使用大肠杆菌K-12野生型和uvrA突变衍生物构建携带一个、两个、三个或更多噬菌体λ cI基因组的同基因菌株，并通过体外操纵子DNA结合试验测量，这些菌株含有浓度不断增加的λ阻遏物。测定了对紫外线照射的存活率和噬菌体诱导情况。在这两种菌株中，均获得了作为细胞阻遏物浓度函数的剂量反应关系。uvrA溶原菌诱导所需的紫外线通量仅为野生型对应物的十分之一。含有过量产生lambdaind+阻遏物的质粒的溶原菌株以及含有过量产生lambdaind-阻遏物的质粒的相同溶原菌株，与非溶原亲本菌株显示出相同的存活曲线；然而，只有前者产生感染中心（辐射后频率为2×10^(-3)至5×10^(-4)）。
相似文献
1
Correlation between UV dose requirement for lambda bacteriophage induction and lambda repressor concentration.λ噬菌体诱导所需紫外线剂量与λ阻遏物浓度之间的相关性。
J Virol. 1978 Jun;26(3):595-602. doi: 10.1128/JVI.26.3.595-602.1978.
2
A model on the induction by UV irradiation of Escherichia coli bacteria lysogenic with the bacteriophage lambda.关于紫外线照射诱导λ噬菌体溶原性大肠杆菌的模型。
Z Allg Mikrobiol. 1974;14(1):63-7.
3
Proteolytic cleavage of bacteriophage lambda repressor in induction.诱导过程中噬菌体λ阻遏物的蛋白水解切割
Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51. doi: 10.1073/pnas.72.1.147.
4
UV-induced alleviation of K-specific restriction of bacteriophage lambda.紫外线诱导的噬菌体λ的K特异性限制的缓解
J Virol. 1977 Mar;21(3):1249-51. doi: 10.1128/JVI.21.3.1249-1251.1977.
5
A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):300-4. doi: 10.1073/pnas.74.1.300.
6
recA+-dependent inactivation of the lambda repressor in Escherichia coli lysogens by gamma-radiation and by tif expression.通过γ辐射和tif表达在大肠杆菌溶原菌中recA +依赖性的λ阻遏物失活
Mol Gen Genet. 1975 Nov 3;141(1):1-8. doi: 10.1007/BF00332374.
7
Recombination of bacteriophage phi X174 by the red function of bacteriophage lambda.通过噬菌体λ的红色功能实现噬菌体φX174的重组。
J Virol. 1979 Feb;29(2):438-42. doi: 10.1128/JVI.29.2.438-442.1979.
8
The influence of prophage lambda on the mutagenic response of Escherichia coli to ultraviolet irradiation.原噬菌体λ对大肠杆菌紫外线诱变反应的影响。
Mutat Res. 1977 Sep;44(3):299-304. doi: 10.1016/0027-5107(77)90089-6.
9
Induction of phage production in the lysogenic Escherichia coli by hydroxyurea.羟基脲诱导溶原性大肠杆菌产生噬菌体
Jpn J Microbiol. 1975 Oct;19(5):349-54. doi: 10.1111/j.1348-0421.1975.tb00891.x.
10
Lysogenic induction of lambdoid phages in lexA mutants of Escherichia coli.大肠杆菌lexA突变体中类λ噬菌体的溶原诱导
Mol Gen Genet. 1981;184(3):457-9. doi: 10.1007/BF00352522.
引用本文的文献
1
High-yield bioproduction of virus-free virus-like P4-EKORhE multi-lysin transducing particles as an antimicrobial gene therapeutic.作为一种抗菌基因疗法，无病毒的病毒样P4-EKORhE多溶素转导颗粒的高产生物生产。
Front Cell Infect Microbiol. 2025 Jun 25;15:1561443. doi: 10.3389/fcimb.2025.1561443. eCollection 2025.
2
Isolation of Bacteriophages from Soil Samples in a Poorly Equipped Field Laboratory in Kruger National Park.从克鲁格国家公园设备简陋的野外实验室的土壤样本中分离噬菌体。
Methods Mol Biol. 2024;2738:91-103. doi: 10.1007/978-1-0716-3549-0_5.
3
Identification of novel prophage regions in genome and gene expression analysis during phage-like particle induction.鉴定基因组中的新型噬菌体区域和噬菌体样颗粒诱导过程中的基因表达分析。
PeerJ. 2022 Feb 15;10:e12956. doi: 10.7717/peerj.12956. eCollection 2022.
4
Noise in timing and precision of gene activities in a genetic cascade.基因级联反应中基因活动时间和精度的噪声。
Mol Syst Biol. 2007;3:71. doi: 10.1038/msb4100113. Epub 2007 Feb 13.
5
Induction of lambdoid prophages by amino acid deprivation: differential inducibility; role of recA.氨基酸剥夺诱导λ样原噬菌体：差异诱导性；recA的作用。
Mol Gen Genet. 1980;180(1):147-55. doi: 10.1007/BF00267364.
6
Induction of prophage lambda without amplification of recA protein.无需recA蛋白扩增即可诱导原噬菌体λ
Mol Gen Genet. 1980;178(2):317-23. doi: 10.1007/BF00270478.
7
Prophage induction in thermosensitive DNA mutants of Bacillus subtilis.枯草芽孢杆菌温度敏感型DNA突变体中的原噬菌体诱导
Mol Gen Genet. 1984;194(3):451-6. doi: 10.1007/BF00425557.
8
Prophage phi 80 is induced in Escherichia coli K12 recA430.原噬菌体φ80在大肠杆菌K12 recA430中被诱导。
Mol Gen Genet. 1983;189(2):199-206. doi: 10.1007/BF00337804.
9
Cleavage of lambda repressor and synthesis of RecA protein induced by transferred UV-damaged F sex factor.转移的紫外线损伤F性因子诱导λ阻遏物的裂解和RecA蛋白的合成。
Mol Gen Genet. 1982;186(2):170-9. doi: 10.1007/BF00331847.
10
Structural analysis of the carboxy terminus of bacteriophage lambda repressor determined by antipeptide antibodies.通过抗肽抗体确定的噬菌体λ阻遏物羧基末端的结构分析
J Bacteriol. 1989 Mar;171(3):1235-44. doi: 10.1128/jb.171.3.1235-1244.1989.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[ON THE MECHANISM OF INDUCTION OF PROPHAGE DEVELOPMENT IN LYSOGENIC BACTERIA].[论溶源性细菌中前噬菌体发育诱导的机制]
C R Hebd Seances Acad Sci. 1964 Jul 20;259:661-4.
3
[Effects of ultraviolet rays on the Escherichia coli K 12 (lambda), K 12 S, lambda lysogenic system].[紫外线对大肠杆菌K12（λ）、K12 S、λ溶原菌系统的影响]
Ann Inst Pasteur (Paris). 1956 Oct;91(4):511-22.
4
Induction of phage development in lysogenic bacteria.溶原性细菌中噬菌体发育的诱导。
Cold Spring Harb Symp Quant Biol. 1953;18:101-21. doi: 10.1101/sqb.1953.018.01.019.
5
The range of action of genes controlling radiation sensitivity in Escherichia coli.控制大肠杆菌辐射敏感性的基因作用范围。
Mutat Res. 1965 Apr;2(2):111-31. doi: 10.1016/0027-5107(65)90042-4.
6
Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda.关于rec+基因在紫外线诱导噬菌体λ原噬菌体过程中作用的转导研究。
J Mol Biol. 1967 Jan 28;23(2):117-33. doi: 10.1016/s0022-2836(67)80021-4.
7
Characteristics of some multiply recombination-deficient strains of Escherichia coli.一些多重重组缺陷型大肠杆菌菌株的特征
J Bacteriol. 1969 Oct;100(1):231-9. doi: 10.1128/jb.100.1.231-239.1969.
8
Breakage of DNA in rec+ and Rec- bacteria by disintegration of radiophosphorus atoms in DNA and possible cause of pleiotropic effects of RecA mutation.DNA中放射性磷原子的衰变导致rec+和Rec-细菌中DNA的断裂以及RecA突变多效性效应的可能原因。
Cold Spring Harb Symp Quant Biol. 1968;33:243-51. doi: 10.1101/sqb.1968.033.01.028.
9
New method for large-scale preparation of covalently closed lambda DNA molecules.大规模制备共价闭合λDNA分子的新方法。
J Virol. 1974 Nov;14(5):1104-7. doi: 10.1128/JVI.14.5.1104-1107.1974.
10
Host DNA replication or excision repair requirement for ultraviolet induction of bacteriophage lambda lysogens.噬菌体λ溶原菌紫外线诱导所需的宿主DNA复制或切除修复
Nature. 1976 May 13;261(5556):164-6. doi: 10.1038/261164a0.
Zotero 插件