通过在DNA过量情况下的竞争杂交对劳氏鼠白血病病毒细胞内RNA基因组进行定量测量。
Quantitative measurement of intracellular RNA genomes of Rauscher murine leukemia virus by competition hybridization in DNA excess.
作者信息
East J L, Chan J C, Bartlett R J, Knesek J E
出版信息
J Virol. 1979 Feb;29(2):818-24. doi: 10.1128/JVI.29.2.818-824.1979.
Abstract
The technique of competition hybridization in DNA excess was used to determine the intracellular distribution of RNA genomes of Rauscher murine leukemia virus. An examination of subcellular RNA fractions revealed that 59% of intracellular viral RNA genomes were associated with the nuclear-enriched fraction, 41% with the cytoplasmic fraction, and 18% with the polysomal-enriched fraction. Also, an analysis of total cellular RNA disclosed that 20% of intracellular viral RNA genomes were polyadenylated and 80% were not polyadenylated.
摘要
采用DNA过量竞争杂交技术来确定劳氏鼠白血病病毒RNA基因组的细胞内分布。对亚细胞RNA组分的检测显示，59%的细胞内病毒RNA基因组与富含细胞核的组分相关，41%与细胞质组分相关，18%与富含多核糖体的组分相关。此外，对总细胞RNA的分析表明，20%的细胞内病毒RNA基因组是聚腺苷酸化的，80%不是聚腺苷酸化的。
相似文献
1
Quantitative measurement of intracellular RNA genomes of Rauscher murine leukemia virus by competition hybridization in DNA excess.
J Virol. 1979 Feb;29(2):818-24. doi: 10.1128/JVI.29.2.818-824.1979.
2
Intracellular distribution and polyadenylate content of adeno-associated virus RNA sequences.
Virology. 1976 Aug;73(1):273-85. doi: 10.1016/0042-6822(76)90080-5.
3
Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells.
J Virol. 1978 Mar;25(3):750-63. doi: 10.1128/JVI.25.3.750-763.1978.
4
[Characteristics of the cellular RNA in viral leukemia].
Vopr Onkol. 1978;24(10):18-23.
5
DNA-RNA hybrid in cells infected by murine leukemia virus.
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1009-14. doi: 10.1101/sqb.1974.039.01.116.
6
Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1093-7. doi: 10.1073/pnas.71.4.1093.
7
The diversity and specificity of nuclear and polysomal poly(A)+ RNA populations in normal and MSV-transformed cells.
Cell Differ. 1977 Jun;6(1):25-39. doi: 10.1016/0045-6039(77)90042-2.
8
Complexity of poly(A+) and poly(A-) polysomal RNA in mouse liver and cultured mouse fibroblasts.
Nucleic Acids Res. 1978 Mar;5(3):697-712. doi: 10.1093/nar/5.3.697.
9
Sequence complexity and abundance classes of nuclear and polysomal polyadenylated RNA in Neurospora crassa.
Biochim Biophys Acta. 1980 Mar 28;607(1):122-35. doi: 10.1016/0005-2787(80)90226-9.
10
5'-terminal nucleotide sequences of the Rauscher leukemia virus and gibbon ape leukemia virus genomes exhibit a high degree of correspondence.
J Virol. 1979 Dec;32(3):803-11. doi: 10.1128/JVI.32.3.803-811.1979.
引用本文的文献
1
Structure of an RNA switch that enforces stringent retroviral genomic RNA dimerization.
Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13640-5. doi: 10.1073/pnas.0606156103. Epub 2006 Aug 31.
2
Characterization of intracellular viral RNA in interferon-treated cells chronically infected with murine leukemia virus.
J Virol. 1980 Sep;35(3):694-703. doi: 10.1128/JVI.35.3.694-703.1980.
本文引用的文献
1
Intracellular and virion 35 S RNA species of murine sarcoma and leukemia viruses.
Virology. 1974 May;59(1):258-65. doi: 10.1016/0042-6822(74)90221-9.
2
RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA.
J Mol Biol. 1973 Oct 15;80(1):93-117. doi: 10.1016/0022-2836(73)90235-0.
3
Messenger RNA in HeLa cells: kinetics of formation and decay.
J Mol Biol. 1973 Aug 5;78(2):321-34. doi: 10.1016/0022-2836(73)90119-8.
4
The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments.
Biochim Biophys Acta. 1972 Dec 22;287(3):485-94. doi: 10.1016/0005-2787(72)90293-6.
5
Nucleotide sequence relationships of avian RNA tumor viruses: measurement of the deletion in a transformation-defective mutant of Rous sarcoma virus.
J Virol. 1974 Apr;13(4):837-46. doi: 10.1128/JVI.13.4.837-846.1974.
6
RD 114 virus-specific sequences in feline cellular RNA: detection and characterization.
J Virol. 1973 Nov;12(5):984-94. doi: 10.1128/JVI.12.5.984-994.1973.
7
Early synthesis of virus-specific RNA and DNA in cells rapidly transformed with Rous sarcoma virus.
Virology. 1973 Dec;56(2):532-48. doi: 10.1016/0042-6822(73)90056-1.
8
Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells.
J Virol. 1973 Apr;11(4):600-2. doi: 10.1128/JVI.11.4.600-602.1973.
9
Virus-specific ribonucleic acid in cells producing rous sarcoma virus: detection and characterization.
J Virol. 1972 Jun;9(6):891-902. doi: 10.1128/JVI.9.6.891-902.1972.
10
Detection of avian tumor virus RNA in uninfected chicken embryo cells.
J Virol. 1973 Feb;11(2):157-67. doi: 10.1128/JVI.11.2.157-167.1973.
Zotero 插件