通过在DNA过量情况下的竞争杂交对劳氏鼠白血病病毒细胞内RNA基因组进行定量测量。
Quantitative measurement of intracellular RNA genomes of Rauscher murine leukemia virus by competition hybridization in DNA excess.
作者信息
East J L, Chan J C, Bartlett R J, Knesek J E
出版信息
J Virol. 1979 Feb;29(2):818-24. doi: 10.1128/JVI.29.2.818-824.1979.
Abstract
The technique of competition hybridization in DNA excess was used to determine the intracellular distribution of RNA genomes of Rauscher murine leukemia virus. An examination of subcellular RNA fractions revealed that 59% of intracellular viral RNA genomes were associated with the nuclear-enriched fraction, 41% with the cytoplasmic fraction, and 18% with the polysomal-enriched fraction. Also, an analysis of total cellular RNA disclosed that 20% of intracellular viral RNA genomes were polyadenylated and 80% were not polyadenylated.
摘要
采用DNA过量竞争杂交技术来确定劳氏鼠白血病病毒RNA基因组的细胞内分布。对亚细胞RNA组分的检测显示，59%的细胞内病毒RNA基因组与富含细胞核的组分相关，41%与细胞质组分相关，18%与富含多核糖体的组分相关。此外，对总细胞RNA的分析表明，20%的细胞内病毒RNA基因组是聚腺苷酸化的，80%不是聚腺苷酸化的。
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