小鼠肝脏和培养的小鼠成纤维细胞中多聚腺苷酸阳性（poly(A+)）和多聚腺苷酸阴性（poly(A-)）多核糖体RNA的复杂性
Complexity of poly(A+) and poly(A-) polysomal RNA in mouse liver and cultured mouse fibroblasts.
作者信息
Grady L J, North A B, Campbell W P
出版信息
Nucleic Acids Res. 1978 Mar;5(3):697-712. doi: 10.1093/nar/5.3.697.
Abstract
RNA excess hybridization experiments were used to measure the complexity of nuclear RNA, poly(A+) mRNA, poly(A-) mRNA, and EDTA-released polysomal RNA sedimenting at less than 80 S in mouse liver and in cultured mouse cells. With both cell types, poly(A-) RNA was found to contain 30-40% of the sequence diversity of total mRNA. In the case of liver this represents 5,700 poly(A-) molecules and 8,600 poly(A+) molecules for a total of approximately 14,300 different mRNAs. Comparison of the complexity of mRNA with that of nuclear RNA revealed that in liver and in cultured cells, mRNA has only 10-20% of the sequence diversity present in nuclear RNA. This latter observation is consistent with existing data on mammalian cells from this and other laboratories.
摘要
RNA过量杂交实验用于测量小鼠肝脏和培养的小鼠细胞中，沉降系数小于80S的核RNA、多聚腺苷酸阳性（poly(A+)）mRNA、多聚腺苷酸阴性（poly(A-)）mRNA以及EDTA释放的多核糖体RNA的复杂度。在这两种细胞类型中，发现poly(A-) RNA包含总mRNA序列多样性的30%-40%。就肝脏而言，这代表5700个poly(A-)分子和8600个poly(A+)分子，总共约14300种不同的mRNA。将mRNA的复杂度与核RNA的复杂度进行比较，发现在肝脏和培养细胞中，mRNA仅具有核RNA中10%-20%的序列多样性。后一观察结果与本实验室和其他实验室关于哺乳动物细胞的现有数据一致。
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