单个脾细胞在完整小鼠干扰素反应中的作用。
The role of individual spleen cells in the interferon response of the intact mouse.
作者信息
Osborn J E, Walker D L
出版信息
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1038-45. doi: 10.1073/pnas.62.4.1038.
A method is described for the detection of interferon production by individual spleen cells of mice after intravenous virus infection. Although mouse spleen plays a major role in the total interferon response to Newcastle disease virus, the number of spleen cells participating is a small fraction of the total, suggesting that interferon formation is to some extent a specialized cell function. Monolayers of mouse embryo cells, after brief contact with agar-suspended spleen cells from interferon-producing mice, have roughly circular foci of cells remaining after removal of agar and vesicular stomatitis virus challenge. The numbers of such foci correlate directly with the number of spleen cells, concentration of inducing virus in the inoculum, duration of contact of cells with mouse-embryo indicator monolayers, time after interferon induction, and serum interferon titer. With this technique, evolution of the interferon-forming cell response in spleens of virus-infected mice has been studied.
本文描述了一种检测静脉注射病毒感染后小鼠单个脾细胞产生干扰素的方法。尽管小鼠脾脏在对新城疫病毒的总体干扰素反应中起主要作用，但参与反应的脾细胞数量仅占总数的一小部分，这表明干扰素的形成在某种程度上是一种特殊的细胞功能。将来自产生干扰素小鼠的琼脂悬浮脾细胞与小鼠胚胎细胞单层短暂接触后，去除琼脂并进行水泡性口炎病毒攻击，剩余的细胞会形成大致圆形的病灶。这些病灶的数量与脾细胞数量、接种物中诱导病毒的浓度、细胞与小鼠胚胎指示单层的接触持续时间、干扰素诱导后的时间以及血清干扰素滴度直接相关。利用这项技术，对病毒感染小鼠脾脏中干扰素形成细胞反应的演变进行了研究。
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