Division of Infectious Diseases, Department of Medicine, Stanford University, Stanford, California 94305.
Infect Immun. 1970 Jul;2(1):69-76. doi: 10.1128/iai.2.1.69-76.1970.
Intraperitoneal injection of mice with mineral oil, incomplete (IFA) or complete Freund's adjuvant (CFA) increased the interferon response to endotoxin or (poly rI)(poly rC) administered intravenously 2 days later. After endotoxin administration, circulating interferon titers were increased at several different times of sampling and with a variety of endotoxin dosages. When injection of endotoxin was delayed until 6 to 8 days after the administration of IFA or CFA, interferon production was markedly decreased. Mice treated with CFA and injected with endotoxin 2 days later became more resistant to intranasal vesicular stomatitis virus challenge than mice injected with endotoxin alone. Hyporeactivity to the interferon-inducing capacity of a second injection of endotoxin 2 days after the first injection could not be overcome by administering CFA simultaneously with the first dose. CFA treatment not only raised the serum interferon titers produced by endotoxin, but also increased the number of interferon-forming cells in the spleen after administration of endotoxin in vivo. In addition, CFA enhanced the intravascular clearance of (poly rI)(poly rC). The possibility that Freund's adjuvant increased the interferon response to endotoxin and (poly rI)*(poly rC) by stimulating the uptake and processing of the interferon inducer by lymphoreticular cells is discussed.
给小鼠腹腔内注射矿物油、不完全弗氏佐剂（IFA）或完全弗氏佐剂（CFA），可增加 2 天后静脉内给予内毒素或（多聚肌苷酸）（多聚胞苷酸）时的干扰素反应。给予内毒素后，在不同的采样时间和不同剂量的内毒素下，循环干扰素滴度均增加。当在给予 IFA 或 CFA 后 6 至 8 天给予内毒素注射时，干扰素的产生明显减少。与单独注射内毒素的小鼠相比，用 CFA 处理并在 2 天后注射内毒素的小鼠对鼻内水疱性口炎病毒攻击的抵抗力更强。在第一次注射后 2 天第二次注射内毒素时，对干扰素诱导能力的低反应性不能通过与第一次剂量同时给予 CFA 来克服。CFA 治疗不仅提高了内毒素产生的血清干扰素滴度，而且在体内给予内毒素后还增加了脾内干扰素形成细胞的数量。此外，CFA 增强了（多聚肌苷酸）（多聚胞苷酸）的血管内清除率。讨论了弗氏佐剂通过刺激淋巴网状细胞摄取和处理干扰素诱导剂来增加对内毒素和（多聚肌苷酸）*（多聚胞苷酸）的干扰素反应的可能性。
