Johnson H M, Brenner K, Hall H E
J Bacteriol. 1969 Oct;100(1):176-9. doi: 10.1128/jb.100.1.176-179.1969.
The type-specific antigen of a strain of Clostridium perfringens involved in food poisoning was isolated from the cell wall by the use of hot formamide. The antigen appears to consist of polysaccharide or mucopeptide. The formamide extract was shown to be heterogeneous by gel filtration on Sephadex G-200. The serologically active fraction contained about 25% of the amount of protein present in the original formamide extract. Hexosamine, acetyl groups, and carbohydrate also were detected. The formamide extract showed a high degree of serological activity. The serological activity was increased twofold on Sephadex gel filtration.
通过使用热甲酰胺从产气荚膜梭菌食物中毒菌株的细胞壁中分离出其型特异性抗原。该抗原似乎由多糖或粘肽组成。经葡聚糖G-200凝胶过滤显示，甲酰胺提取物具有异质性。血清学活性部分所含蛋白质约为原始甲酰胺提取物中蛋白质含量的25%。还检测到己糖胺、乙酰基和碳水化合物。甲酰胺提取物显示出高度的血清学活性。经葡聚糖凝胶过滤后，血清学活性增加了两倍。
