Dayalu K I, Cherniak R, Hatheway C L
Infect Immun. 1981 Feb;31(2):608-14. doi: 10.1128/iai.31.2.608-614.1981.
Soluble antigens were obtained by extracting five serotype strains of Clostridium perfringens type A with water at 100 degrees C. The type-specific polysaccharides were precipitated with ethanol, and the common antigens were recovered from the ethanol supernatants by concentration, dialysis, and lyophilization. Refluxing the water-extracted cell residues with 1% acetic acid followed by concentration, dialysis, and lyophilization gave additional common antigen fractions. A comprehensive, side-by-side comparison of the antigen fractions, the ethanol precipitate, the ethanol supernatant, and the acetic acid supernatant, revealed that common antigens were recovered in all three fractions, and that three distinct entities were responsible for the formation of the observed common immunoprecipitin lines; whereas many fractions possessed all three immunoprecipitin lines, others contained only one or two. The serological homology observed between the various antigen fractions was apparently a consequence of N-acetylglucosamine- and N-acetylmannosamine-containing polymers. The common antigens were presumably associated with the cell envelope and may be the type of markers sought previously by others for the serological identification of C. perfringens.
通过在100℃用水提取五株A型产气荚膜梭菌血清型菌株获得可溶性抗原。用乙醇沉淀型特异性多糖，通过浓缩、透析和冻干从乙醇上清液中回收共同抗原。用水提取的细胞残渣与1%乙酸回流，然后进行浓缩、透析和冻干，得到额外的共同抗原组分。对抗原组分、乙醇沉淀物、乙醇上清液和乙酸上清液进行全面的并排比较，发现所有三个组分中都回收了共同抗原，并且有三种不同的实体负责观察到的共同免疫沉淀线的形成；虽然许多组分具有所有三条免疫沉淀线，但其他组分只含有一条或两条。在各种抗原组分之间观察到的血清学同源性显然是含N-乙酰葡糖胺和N-乙酰甘露糖胺聚合物的结果。共同抗原可能与细胞包膜相关，可能是其他人先前寻求的用于产气荚膜梭菌血清学鉴定的标记物类型。
