Robinson B H, Halperin M L
Biochem J. 1970 Jan;116(2):229-33. doi: 10.1042/bj1160229.
Mitochondria from rat white adipose tissue were prepared, exhibiting good respiratory control and P/O ratios. They would not oxidize NADH unless NNN'N'-tetramethyl-p-phenylenediamine was added as a carrier of reducing equivalents. These mitochondria were found to oxidize neither l-glycerol 3-phosphate nor l-glutamate plus l-malate at significant rates. The activity of aspartate aminotransferase in these mitochondria was found to be low compared with that found in rat liver mitochondria. As a consequence of this, the adipose-tissue mitochondria exhibited very low rates of cytoplasmic NADH oxidation in a reconstituted Borst (1962) cycle compared with liver mitochondria.
制备了来自大鼠白色脂肪组织的线粒体,其表现出良好的呼吸控制和P/O比值。除非添加NNN'N'-四甲基对苯二胺作为还原当量的载体,否则它们不会氧化NADH。发现这些线粒体既不能以显著速率氧化3-磷酸甘油,也不能氧化谷氨酸加苹果酸。与大鼠肝脏线粒体相比,发现这些线粒体中天冬氨酸转氨酶的活性较低。因此,与肝脏线粒体相比,在重构的博斯特(1962年)循环中,脂肪组织线粒体的细胞质NADH氧化速率非常低。