Peters J H
Microsc Acta. 1979 Jan;81(3):217-25.
Optical components of an inverted microscope have been utilized to set up a device for the observation of living cells under phase contrast conditions. Time lapse recording is performed either by use of a super-8 movie-camera or by a 1/2 inch video device. A special design provides a high focal stability. Standard plastic culture flasks can be used for high optical quality recordings. Both types of recording require low light intensities which results in a minimal light induced cell damaging.
倒置显微镜的光学部件已被用于搭建一种在相差条件下观察活细胞的装置。延时记录可通过使用超8电影摄像机或1/2英寸视频设备来进行。一种特殊设计提供了高聚焦稳定性。标准塑料培养瓶可用于高质量的光学记录。两种记录方式都需要低光强度,这使得光诱导的细胞损伤最小化。