球形红假单胞菌提取物中δ-氨基乙酰丙酸合成酶的激活
Activation of delta-aminolaevulate synthetase in extracts of Rhodopseudomonas spheroides.
作者信息
Marriott J, Neuberger A, Tait G H
出版信息
Biochem J. 1970 Apr;117(3):609-13. doi: 10.1042/bj1170609.
Abstract
- The spontaneous activation of delta-aminolaevulate synthetase in extracts from Rhodopseudomonas spheroides grown semi-anaerobically in the light requires oxygen and does not take place anaerobically in the dark. 2. Activation is completely prevented by azide or cyanide and is partially inhibited by chlorpromazine. These compounds inhibit markedly the succinoxidase activity of extracts. 3. NADH delays activation, but when it has been oxidized by the extract activation begins at the normal rate and complete activation occurs. By contrast both the rate and the extent of activation are markedly decreased by even small amounts of carboxylic acids. 4. The inhibitory effects of succinate and citrate on activation can be prevented by malonate and fluorocitrate respectively. 5. These results suggest that for activation to occur some endogenous compound has to be oxidized via the electron transport chain. 6. Activation occurs under anaerobic conditions in the light, probably by photo-oxidation.
摘要
- 在光照下半厌氧培养的球形红假单胞菌提取物中，δ-氨基乙酰丙酸合成酶的自发激活需要氧气，在黑暗中厌氧条件下不会发生。2. 叠氮化物或氰化物可完全阻止激活，氯丙嗪可部分抑制激活。这些化合物显著抑制提取物的琥珀酸氧化酶活性。3. NADH会延迟激活，但当它被提取物氧化后，激活以正常速率开始并完全激活。相比之下，即使少量的羧酸也会显著降低激活的速率和程度。4. 琥珀酸和柠檬酸对激活的抑制作用可分别被丙二酸和氟柠檬酸阻止。5. 这些结果表明，为了发生激活，一些内源性化合物必须通过电子传递链被氧化。6. 在光照下的厌氧条件下会发生激活，可能是通过光氧化作用。
相似文献
1
Activation of delta-aminolaevulate synthetase in extracts of Rhodopseudomonas spheroides.球形红假单胞菌提取物中δ-氨基乙酰丙酸合成酶的激活
Biochem J. 1970 Apr;117(3):609-13. doi: 10.1042/bj1170609.
2
Control of delta-aminolaevulate synthetase activity in Rhodopseudomonas spheroides.球形红假单胞菌中δ-氨基乙酰丙酸合成酶活性的调控
Biochem J. 1969 Feb;111(4):385-94. doi: 10.1042/bj1110385.
3
Change of metabolic stability of delta-aminolevulinate synthetase in Rhodopseudomonas spheroides cells probably related to changes of intracellular oxidation-reduction state.
J Biochem. 1968 Dec;64(6):795-805. doi: 10.1093/oxfordjournals.jbchem.a128962.
4
Occurrence of a specific and reversible inhibitor of delta-aminolevulinate synthetase in extracts of Rhodopseudomonas spheroides.
Arch Biochem Biophys. 1969 Mar;130(1):92-100. doi: 10.1016/0003-9861(69)90014-9.
5
Differential induction of fraction I and fraction II of delta-aminolevulinate synthetase in Rhodopseudomonas spheroides under various incubation conditions.
Arch Biochem Biophys. 1970 May;138(1):155-9. doi: 10.1016/0003-9861(70)90294-8.
6
7
A sensitive radiochemical assay method for delta-aminolevulinic acid synthetase.一种用于δ-氨基乙酰丙酸合成酶的灵敏放射化学测定方法。
J Biol Chem. 1969 Jan 10;244(1):60-7.
8
COFACTOR-DEPENDENT ALDOSE DEHYDROGENASE OF RHODOPSEUDOMONAS SPHEROIDES.球形红假单胞菌的辅因子依赖性醛糖脱氢酶
J Bacteriol. 1965 Mar;89(3):697-705. doi: 10.1128/jb.89.3.697-705.1965.
9
Differential inhibition of induced syntheses of delta-aminolevulinate synthetase and bacteriochlorophyll in dark-aerobically grown Rhodopseudomonas spheroides.
J Biochem. 1967 Feb;61(2):186-92. doi: 10.1093/oxfordjournals.jbchem.a128530.
10
[Oxidative phosphorylation capacity of Rhodopseudomonas palustris during growth in light and darkness].[沼泽红假单胞菌在光照和黑暗条件下生长期间的氧化磷酸化能力]
Mikrobiologiia. 1976 Mar-Apr;45(2):197-200.
引用本文的文献
1
Measurement of delta-aminolevulinic acid synthetase activity in human erythroblasts.人成红细胞中δ-氨基乙酰丙酸合成酶活性的测定。
J Clin Invest. 1974 May;53(5):1326-34. doi: 10.1172/JCI107680.
2
Aminolaevulinate synthetase of Micrococcus denitrificans. Purification and properties of the enzyme, and the effect of growth conditions on the enzyme activity in cells.反硝化微球菌的氨基乙酰丙酸合成酶。该酶的纯化及性质，以及生长条件对细胞中酶活性的影响。
Biochem J. 1973 Feb;131(2):389-403. doi: 10.1042/bj1310389.
3
Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides. The purification and properties of an endogenous activator of the enzyme.球形红假单胞菌中5-氨基乙酰丙酸合成酶活性的调控。该酶内源性激活剂的纯化及性质
Biochem J. 1973 Nov;136(3):491-9. doi: 10.1042/bj1360491.
4
Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides. The involvement of sulphur metabolism.球形红假单胞菌中5-氨基乙酰丙酸合成酶活性的调控。硫代谢的参与。
Biochem J. 1973 Nov;136(3):477-90. doi: 10.1042/bj1360477.
5
[The influence of culture conditions on the NAD(P) content of Rhodospirillum rubrum cells].[培养条件对深红红螺菌细胞中NAD(P)含量的影响]
Arch Mikrobiol. 1971;79(2):147-63.
6
Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides a role for trisulphides.球形红假单胞菌中5-氨基乙酰丙酸合成酶活性的调控：三硫化物的作用
Biochem J. 1975 Aug;150(2):245-57. doi: 10.1042/bj1500245.
本文引用的文献
1
The effect of chlorpromazine on the respiratory chain; cytochrome oxidase.氯丙嗪对呼吸链；细胞色素氧化酶的作用。
Biochem J. 1959 Jun;72(2):204-9. doi: 10.1042/bj0720204.
2
Control of delta-aminolaevulate synthetase activity in Rhodopseudomonas spheroides.球形红假单胞菌中δ-氨基乙酰丙酸合成酶活性的调控
Biochem J. 1969 Feb;111(4):385-94. doi: 10.1042/bj1110385.
3
Regulation of porphyrin synthesis.卟啉合成的调节
Biochem Soc Symp. 1968;28:61-74.
Zotero 插件