Casto B C
J Virol. 1968 Apr;2(4):376-83. doi: 10.1128/JVI.2.4.376-383.1968.
Inoculation of hamster embryo cell cultures with human adenovirus type 12 (Ad12) or simian adenovirus (SA7) resulted in the formation of foci of morphologically transformed cells within 12 days. The rapid appearance of well-defined foci was dependent upon the transfer of cells into new plates, with sufficient dilution after virus adsorption, and was independent of virus dose. Dose-response studies showed linearity of focus formation with dilution of Ad12 or SA7. Results averaged from several experiments show plaque-forming unit to focus-forming unit ratios of approximately 1.8 x 10(6) for Ad12 and 2.6 x 10(5) for SA7. Other experiments showed that most of the adenovirus involved in transformation was adsorbed by 3 hr. Cell lines derived from SA7 transformed cells produced tumors within 19 days when inoculated intradermally into young adult hamsters. Such cell-induced tumors histologically resembled SA7 virus-induced hamster tumors. Formation of tumors with SA7 transformed cells was inhibited by prior immunization of test animals with SA7 or Ad12 virus.
用人腺病毒12型（Ad12）或猴腺病毒（SA7）接种仓鼠胚胎细胞培养物，12天内形成了形态转化细胞灶。界限分明的病灶迅速出现，这取决于细胞转移到新平板中，病毒吸附后进行充分稀释，且与病毒剂量无关。剂量反应研究表明，Ad12或SA7稀释后，病灶形成呈线性关系。几个实验的平均结果显示，Ad12的噬斑形成单位与病灶形成单位之比约为1.8×10⁶，SA7为2.6×10⁵。其他实验表明，参与转化的大多数腺病毒在3小时内被吸附。从SA7转化细胞衍生的细胞系，当皮内接种到年轻成年仓鼠体内时，19天内会产生肿瘤。这种细胞诱导的肿瘤在组织学上类似于SA7病毒诱导的仓鼠肿瘤。用SA7或Ad12病毒预先免疫实验动物，可抑制SA7转化细胞形成肿瘤。
