Casto B C
J Virol. 1969 May;3(5):513-9. doi: 10.1128/JVI.3.5.513-519.1969.
Simian adenovirus, SV11, readily transformed hamster embryo cell cultures in vitro and produced tumors in vivo when inoculated into newborn hamsters. Foci consisting of small, loosely attached, rounded cells could be seen as early as 7 days postinoculation. Many of these cells contained several nuclei or the nucleus was multilobed. The cells grew without extensive cell to cell contact or formed small chains or clusters when passaged in vitro. This pattern of cell morphology and growth has not been reported with other simian or human adenovirus-transformed cells. Linearity of foci formation with virus dilution was observed when the virus multiplicity was less than 3 plaque-forming units (PFU)/cell. The PFU to focus-forming units ratio for SV11 was found to be 2 x 10(4) to 4 x 10(4), which is approximately 5- to 10-fold and 50- to 100-fold lower than those reported for simian adenovirus, SA7, and human adenovirus type 12, respectively. Cells transformed by SV11: (i) produced tumors when inoculated into young hamsters, (ii) contained tumor antigen which reacts with serum obtained from hamsters bearing SV11 passaged tumors, and (iii) could be propagated in vitro through an indefinite number of generations.
猿猴腺病毒SV11在体外能轻易转化仓鼠胚胎细胞培养物，接种到新生仓鼠体内时可产生肿瘤。早在接种后7天就能看到由小的、附着松散的圆形细胞组成的病灶。这些细胞中的许多含有几个细胞核，或者细胞核呈多叶状。这些细胞生长时细胞间接触不广泛，在体外传代时形成小链或小簇。其他猿猴或人腺病毒转化的细胞尚未报道过这种细胞形态和生长模式。当病毒复数小于3个空斑形成单位（PFU）/细胞时，观察到病灶形成与病毒稀释呈线性关系。发现SV11的PFU与病灶形成单位的比率为2×10⁴至4×10⁴，分别比猿猴腺病毒SA7和人腺病毒12型报道的比率低约5至10倍和50至100倍。由SV11转化的细胞：（i）接种到幼仓鼠体内时产生肿瘤，（ii）含有与从携带SV11传代肿瘤的仓鼠获得的血清发生反应的肿瘤抗原，（iii）可在体外无限传代繁殖。
