自旋标记的粗糙脉孢菌线粒体。
Spin-labeled Neurospora mitochondria.
作者信息
Keith A, Bulfield G, Snipes W
出版信息
Biophys J. 1970 Jul;10(7):618-29. doi: 10.1016/S0006-3495(70)86324-X.
Abstract
Spin-label studies were carried out on Neurospora mitochondria under in vivo and in vitro labeling conditions. A long-chained spin-labeled fatty acid was incorporated by Neurospora and was found in mitochondrial phospholipids. The molecular motion at various temperatures was different from that for the same spin label under in vitro labeling conditions. The results for spin-labeled mitochondria were compared with those from isolated lipids and with those from aggregates of spin-labeled fatty acid and isolated bovine serum albumin. These comparisons suggest that the hydrocarbon portions of membranes are relatively fluid and are not extensively restricted in motion by association with proteins.
摘要
在体内和体外标记条件下，对粗糙脉孢菌线粒体进行了自旋标记研究。粗糙脉孢菌掺入了一种长链自旋标记脂肪酸，并在线粒体磷脂中发现了该脂肪酸。在不同温度下的分子运动与体外标记条件下相同自旋标记的分子运动不同。将自旋标记线粒体的结果与分离脂质的结果以及自旋标记脂肪酸与分离牛血清白蛋白聚集体的结果进行了比较。这些比较表明，膜的烃部分相对流体，并且通过与蛋白质结合在运动中没有受到广泛限制。
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