兼性嗜热芽孢杆菌细胞膜的自旋标记研究
Spin-labeling studies on the membrane of a facultative thermophilic bacillus.
作者信息
Chan M, Virmani Y P, Himes R H, Akagi J M
出版信息
J Bacteriol. 1973 Jan;113(1):322-8. doi: 10.1128/jb.113.1.322-328.1973.
Abstract
Spin-labeling studies were conducted to elucidate the rigidity and viscosity of the membrane of a facultative thermophilic bacillus. By employing nitroxide stearate probes, it was observed that this fatty acid was incorporated into the membrane fraction. Electron spin resonance spectroscopy indicated that cells grown at 55 C contained a membrane which was more rigid and viscous than the membrane from cells grown at 37 C.
摘要
进行自旋标记研究以阐明兼性嗜热芽孢杆菌膜的刚性和粘度。通过使用硬脂酸氮氧化物探针，观察到这种脂肪酸被整合到膜组分中。电子自旋共振光谱表明，在55℃下生长的细胞所含的膜比在37℃下生长的细胞的膜更刚性且更具粘性。
相似文献
1
Spin-labeling studies on the membrane of a facultative thermophilic bacillus.兼性嗜热芽孢杆菌细胞膜的自旋标记研究
J Bacteriol. 1973 Jan;113(1):322-8. doi: 10.1128/jb.113.1.322-328.1973.
2
Tumor lipids: characterization of the lipids isolated from membranous material.肿瘤脂质：从膜性物质中分离出的脂质的特性
Arch Biochem Biophys. 1970 Nov;141(1):190-7. doi: 10.1016/0003-9861(70)90122-0.
3
Temperature-dependent lipid content and fatty acid composition of three thermophilic bacteria.三种嗜热细菌的温度依赖性脂质含量和脂肪酸组成
Antonie Van Leeuwenhoek. 1985;51(2):155-65. doi: 10.1007/BF02310009.
4
Alterations in normal fatty acid composition in a temperature-sensitive mutant of a thermophilic bacillus.嗜热芽孢杆菌温度敏感突变体中正常脂肪酸组成的改变。
Arch Microbiol. 1974 Apr 19;97(2):89-102. doi: 10.1007/BF00403049.
5
Membrane lipids of Mycoplasma hominis.人型支原体的膜脂
J Bacteriol. 1973 Feb;113(2):565-71. doi: 10.1128/jb.113.2.565-571.1973.
6
Fatty acid composition of lipid extracts of a thermophilic Bacillus species.一种嗜热芽孢杆菌属物种脂质提取物的脂肪酸组成。
J Bacteriol. 1970 Jan;101(1):145-51. doi: 10.1128/jb.101.1.145-151.1970.
7
Saturated fatty acid requirer of Neurospora crassa.粗糙脉孢菌的饱和脂肪酸需求者。
J Bacteriol. 1971 Apr;106(1):174-82. doi: 10.1128/jb.106.1.174-182.1971.
8
Lipids of alcoholophilic lactobacilli. II. Occurrence of polar lipids with unusually long acyl chains in Lactobacillus heterohiochii.嗜酒精乳杆菌的脂质。II. 嗜异源乳杆菌中具有异常长酰基链的极性脂质的存在情况。
Biochim Biophys Acta. 1974 Nov 18;369(2):146-55. doi: 10.1016/0005-2760(74)90248-3.
9
Phospholipid class and fatty acid composition of golgi apparatus isolated from rat liver and comparison with other cell fractions.从大鼠肝脏分离的高尔基体的磷脂类别和脂肪酸组成及其与其他细胞组分的比较。
Biochemistry. 1970 Jan 6;9(1):19-25. doi: 10.1021/bi00803a003.
10
Effect of growth temperature on membrane dynamics in a thermophilic cyanobacterium: a spin label study.生长温度对嗜热蓝细菌膜动力学的影响：一项自旋标记研究。
Biochim Biophys Acta. 1988 Sep 1;943(3):501-10. doi: 10.1016/0005-2736(88)90383-5.
引用本文的文献
1
Microbial biomass, activity, and community structure of water and particulates retrieved by backflow from a waterflood injection well.从注水注水井回流的水和颗粒中回收的微生物生物量、活性和群落结构。
Appl Environ Microbiol. 1988 Jun;54(6):1383-93. doi: 10.1128/aem.54.6.1383-1393.1988.
2
The effect of temperature on the growth and lipid composition of the extremely halophilic coccus, Sarcina marina.温度对极端嗜盐球菌——海八叠球菌生长和脂质组成的影响
Antonie Van Leeuwenhoek. 1981 Mar;47(1):25-40. doi: 10.1007/BF00399064.
3
Correlation between thermal death and membrane fluidity in Bacillus stearothermophilus.嗜热脂肪芽孢杆菌热致死与膜流动性之间的相关性
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4111-5. doi: 10.1073/pnas.71.10.4111.
4
Membranes of Bacillus stearothermophilus: factors affecting protoplast stability and thermostability of alkaline phosphatase and reduced nicotinamide adenine dinucleotide oxidase.嗜热脂肪芽孢杆菌的膜：影响原生质体稳定性以及碱性磷酸酶和还原型烟酰胺腺嘌呤二核苷酸氧化酶热稳定性的因素
J Bacteriol. 1973 Jun;114(3):1336-45. doi: 10.1128/jb.114.3.1336-1345.1973.
5
Spin-labeling studies on the lipids of psychrophilic, psychrotrophic, and mesophilic clostridia.嗜冷、嗜冷兼嗜温及嗜温梭菌脂质的自旋标记研究
J Bacteriol. 1976 Jan;125(1):211-9. doi: 10.1128/jb.125.1.211-219.1976.
6
Lipid and protein composition of membranes of Bacillus megaterium variants in the temperature range 5 to 70 degrees C.巨大芽孢杆菌变种在5至70摄氏度温度范围内细胞膜的脂质和蛋白质组成
J Bacteriol. 1978 Sep;135(3):1043-52. doi: 10.1128/jb.135.3.1043-1052.1978.
本文引用的文献
1
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
2
Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography.气液色谱法测定超微脂质样品中的脂肪酸含量及组成
J Lipid Res. 1966 May;7(3):442-5.
3
Fatty acid composition of thermophilic, mesophilic, and psychrophilic clostridia.嗜热、嗜温及嗜冷梭菌的脂肪酸组成
J Bacteriol. 1971 Jun;106(3):876-81. doi: 10.1128/jb.106.3.876-881.1971.
4
Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus.芽孢杆菌属嗜温菌和嗜热菌中的脂肪酸分布
J Bacteriol. 1970 Aug;103(2):479-81. doi: 10.1128/jb.103.2.479-481.1970.
5
Physics and chemistry of spin labels.自旋标记物的物理与化学
Q Rev Biophys. 1970 Feb;3(1):91-136. doi: 10.1017/s003358350000442x.
6
Motion of fatty acid spin labels in the plasma membrane of mycoplasma.
Biochim Biophys Acta. 1970;219(1):104-13. doi: 10.1016/0005-2736(70)90065-9.
7
Spin-labeled Neurospora mitochondria.自旋标记的粗糙脉孢菌线粒体。
Biophys J. 1970 Jul;10(7):618-29. doi: 10.1016/S0006-3495(70)86324-X.
8
Orientation and motion of amphiphilic spin labels in membranes.两亲性自旋标记物在膜中的取向和运动
Proc Natl Acad Sci U S A. 1969 Sep;64(1):20-7. doi: 10.1073/pnas.64.1.20.
9
Spin-label studies of the excitable membranes of nerve and muscle.神经和肌肉可兴奋膜的自旋标记研究。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):12-6. doi: 10.1073/pnas.61.1.12.
10
Spin-labeled mitochondrial lipids in Neurospora crassa.粗糙脉孢菌中的自旋标记线粒体脂质。
Proc Natl Acad Sci U S A. 1968 Nov;61(3):819-26. doi: 10.1073/pnas.61.3.819.
Zotero 插件