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1
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J Bacteriol. 1973 Jan;113(1):322-8. doi: 10.1128/jb.113.1.322-328.1973.
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Alterations in normal fatty acid composition in a temperature-sensitive mutant of a thermophilic bacillus.嗜热芽孢杆菌温度敏感突变体中正常脂肪酸组成的改变。
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J Bacteriol. 1971 Apr;106(1):174-82. doi: 10.1128/jb.106.1.174-182.1971.
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Effect of growth temperature on membrane dynamics in a thermophilic cyanobacterium: a spin label study.生长温度对嗜热蓝细菌膜动力学的影响:一项自旋标记研究。
Biochim Biophys Acta. 1988 Sep 1;943(3):501-10. doi: 10.1016/0005-2736(88)90383-5.

引用本文的文献

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Appl Environ Microbiol. 1988 Jun;54(6):1383-93. doi: 10.1128/aem.54.6.1383-1393.1988.
2
The effect of temperature on the growth and lipid composition of the extremely halophilic coccus, Sarcina marina.温度对极端嗜盐球菌——海八叠球菌生长和脂质组成的影响
Antonie Van Leeuwenhoek. 1981 Mar;47(1):25-40. doi: 10.1007/BF00399064.
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Correlation between thermal death and membrane fluidity in Bacillus stearothermophilus.嗜热脂肪芽孢杆菌热致死与膜流动性之间的相关性
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4
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J Bacteriol. 1976 Jan;125(1):211-9. doi: 10.1128/jb.125.1.211-219.1976.
6
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J Bacteriol. 1978 Sep;135(3):1043-52. doi: 10.1128/jb.135.3.1043-1052.1978.

本文引用的文献

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A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
2
Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography.气液色谱法测定超微脂质样品中的脂肪酸含量及组成
J Lipid Res. 1966 May;7(3):442-5.
3
Fatty acid composition of thermophilic, mesophilic, and psychrophilic clostridia.嗜热、嗜温及嗜冷梭菌的脂肪酸组成
J Bacteriol. 1971 Jun;106(3):876-81. doi: 10.1128/jb.106.3.876-881.1971.
4
Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus.芽孢杆菌属嗜温菌和嗜热菌中的脂肪酸分布
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Physics and chemistry of spin labels.自旋标记物的物理与化学
Q Rev Biophys. 1970 Feb;3(1):91-136. doi: 10.1017/s003358350000442x.
6
Motion of fatty acid spin labels in the plasma membrane of mycoplasma.
Biochim Biophys Acta. 1970;219(1):104-13. doi: 10.1016/0005-2736(70)90065-9.
7
Spin-labeled Neurospora mitochondria.自旋标记的粗糙脉孢菌线粒体。
Biophys J. 1970 Jul;10(7):618-29. doi: 10.1016/S0006-3495(70)86324-X.
8
Orientation and motion of amphiphilic spin labels in membranes.两亲性自旋标记物在膜中的取向和运动
Proc Natl Acad Sci U S A. 1969 Sep;64(1):20-7. doi: 10.1073/pnas.64.1.20.
9
Spin-label studies of the excitable membranes of nerve and muscle.神经和肌肉可兴奋膜的自旋标记研究。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):12-6. doi: 10.1073/pnas.61.1.12.
10
Spin-labeled mitochondrial lipids in Neurospora crassa.粗糙脉孢菌中的自旋标记线粒体脂质。
Proc Natl Acad Sci U S A. 1968 Nov;61(3):819-26. doi: 10.1073/pnas.61.3.819.

兼性嗜热芽孢杆菌细胞膜的自旋标记研究

Spin-labeling studies on the membrane of a facultative thermophilic bacillus.

作者信息

Chan M, Virmani Y P, Himes R H, Akagi J M

出版信息

J Bacteriol. 1973 Jan;113(1):322-8. doi: 10.1128/jb.113.1.322-328.1973.

DOI:10.1128/jb.113.1.322-328.1973
PMID:4347246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC251634/
Abstract

Spin-labeling studies were conducted to elucidate the rigidity and viscosity of the membrane of a facultative thermophilic bacillus. By employing nitroxide stearate probes, it was observed that this fatty acid was incorporated into the membrane fraction. Electron spin resonance spectroscopy indicated that cells grown at 55 C contained a membrane which was more rigid and viscous than the membrane from cells grown at 37 C.

摘要

进行自旋标记研究以阐明兼性嗜热芽孢杆菌膜的刚性和粘度。通过使用硬脂酸氮氧化物探针,观察到这种脂肪酸被整合到膜组分中。电子自旋共振光谱表明,在55℃下生长的细胞所含的膜比在37℃下生长的细胞的膜更刚性且更具粘性。